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Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan
A/Narita/1/2009 (A/N) was the first H1N1 virus from the 2009 pandemic (H1pdm) to be isolated in Japan. To better understand and predict the possible development of this virus strain, the effect of passaging A/N was investigated in Madin-Darby canine kidney cells, chicken eggs and mice. A/N that had...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4469301/ https://www.ncbi.nlm.nih.gov/pubmed/26079133 http://dx.doi.org/10.1371/journal.pone.0130208 |
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author | Ainai, Akira Hasegawa, Hideki Obuchi, Masatsugu Odagiri, Takato Ujike, Makoto Shirakura, Masayuki Nobusawa, Eri Tashiro, Masato Asanuma, Hideki |
author_facet | Ainai, Akira Hasegawa, Hideki Obuchi, Masatsugu Odagiri, Takato Ujike, Makoto Shirakura, Masayuki Nobusawa, Eri Tashiro, Masato Asanuma, Hideki |
author_sort | Ainai, Akira |
collection | PubMed |
description | A/Narita/1/2009 (A/N) was the first H1N1 virus from the 2009 pandemic (H1pdm) to be isolated in Japan. To better understand and predict the possible development of this virus strain, the effect of passaging A/N was investigated in Madin-Darby canine kidney cells, chicken eggs and mice. A/N that had been continuously passaged in cells, eggs, or mice obtained the ability to grow efficiently in each host. Moreover, A/N grown in mice had both a high level of pathogenicity in mice and an increased growth rate in cells and eggs. Changes in growth and pathogenicity were accompanied by amino acid substitutions in viral hemagglutinin (HA) and PB2. In addition, the adapted viruses exhibited a reduced ability to react with ferret antisera against A/N. In conclusion, prolonged passaging allowed influenza A/N to adapt to different hosts, as indicated by a high increase in proliferative capacity that was accompanied by an antigenic alteration leading to amino acid substitutions. |
format | Online Article Text |
id | pubmed-4469301 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44693012015-06-22 Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan Ainai, Akira Hasegawa, Hideki Obuchi, Masatsugu Odagiri, Takato Ujike, Makoto Shirakura, Masayuki Nobusawa, Eri Tashiro, Masato Asanuma, Hideki PLoS One Research Article A/Narita/1/2009 (A/N) was the first H1N1 virus from the 2009 pandemic (H1pdm) to be isolated in Japan. To better understand and predict the possible development of this virus strain, the effect of passaging A/N was investigated in Madin-Darby canine kidney cells, chicken eggs and mice. A/N that had been continuously passaged in cells, eggs, or mice obtained the ability to grow efficiently in each host. Moreover, A/N grown in mice had both a high level of pathogenicity in mice and an increased growth rate in cells and eggs. Changes in growth and pathogenicity were accompanied by amino acid substitutions in viral hemagglutinin (HA) and PB2. In addition, the adapted viruses exhibited a reduced ability to react with ferret antisera against A/N. In conclusion, prolonged passaging allowed influenza A/N to adapt to different hosts, as indicated by a high increase in proliferative capacity that was accompanied by an antigenic alteration leading to amino acid substitutions. Public Library of Science 2015-06-16 /pmc/articles/PMC4469301/ /pubmed/26079133 http://dx.doi.org/10.1371/journal.pone.0130208 Text en © 2015 Ainai et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ainai, Akira Hasegawa, Hideki Obuchi, Masatsugu Odagiri, Takato Ujike, Makoto Shirakura, Masayuki Nobusawa, Eri Tashiro, Masato Asanuma, Hideki Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan |
title | Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan |
title_full | Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan |
title_fullStr | Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan |
title_full_unstemmed | Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan |
title_short | Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan |
title_sort | host adaptation and the alteration of viral properties of the first influenza a/h1n1pdm09 virus isolated in japan |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4469301/ https://www.ncbi.nlm.nih.gov/pubmed/26079133 http://dx.doi.org/10.1371/journal.pone.0130208 |
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