Cargando…

Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan

A/Narita/1/2009 (A/N) was the first H1N1 virus from the 2009 pandemic (H1pdm) to be isolated in Japan. To better understand and predict the possible development of this virus strain, the effect of passaging A/N was investigated in Madin-Darby canine kidney cells, chicken eggs and mice. A/N that had...

Descripción completa

Detalles Bibliográficos
Autores principales: Ainai, Akira, Hasegawa, Hideki, Obuchi, Masatsugu, Odagiri, Takato, Ujike, Makoto, Shirakura, Masayuki, Nobusawa, Eri, Tashiro, Masato, Asanuma, Hideki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4469301/
https://www.ncbi.nlm.nih.gov/pubmed/26079133
http://dx.doi.org/10.1371/journal.pone.0130208
_version_ 1782376607028084736
author Ainai, Akira
Hasegawa, Hideki
Obuchi, Masatsugu
Odagiri, Takato
Ujike, Makoto
Shirakura, Masayuki
Nobusawa, Eri
Tashiro, Masato
Asanuma, Hideki
author_facet Ainai, Akira
Hasegawa, Hideki
Obuchi, Masatsugu
Odagiri, Takato
Ujike, Makoto
Shirakura, Masayuki
Nobusawa, Eri
Tashiro, Masato
Asanuma, Hideki
author_sort Ainai, Akira
collection PubMed
description A/Narita/1/2009 (A/N) was the first H1N1 virus from the 2009 pandemic (H1pdm) to be isolated in Japan. To better understand and predict the possible development of this virus strain, the effect of passaging A/N was investigated in Madin-Darby canine kidney cells, chicken eggs and mice. A/N that had been continuously passaged in cells, eggs, or mice obtained the ability to grow efficiently in each host. Moreover, A/N grown in mice had both a high level of pathogenicity in mice and an increased growth rate in cells and eggs. Changes in growth and pathogenicity were accompanied by amino acid substitutions in viral hemagglutinin (HA) and PB2. In addition, the adapted viruses exhibited a reduced ability to react with ferret antisera against A/N. In conclusion, prolonged passaging allowed influenza A/N to adapt to different hosts, as indicated by a high increase in proliferative capacity that was accompanied by an antigenic alteration leading to amino acid substitutions.
format Online
Article
Text
id pubmed-4469301
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-44693012015-06-22 Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan Ainai, Akira Hasegawa, Hideki Obuchi, Masatsugu Odagiri, Takato Ujike, Makoto Shirakura, Masayuki Nobusawa, Eri Tashiro, Masato Asanuma, Hideki PLoS One Research Article A/Narita/1/2009 (A/N) was the first H1N1 virus from the 2009 pandemic (H1pdm) to be isolated in Japan. To better understand and predict the possible development of this virus strain, the effect of passaging A/N was investigated in Madin-Darby canine kidney cells, chicken eggs and mice. A/N that had been continuously passaged in cells, eggs, or mice obtained the ability to grow efficiently in each host. Moreover, A/N grown in mice had both a high level of pathogenicity in mice and an increased growth rate in cells and eggs. Changes in growth and pathogenicity were accompanied by amino acid substitutions in viral hemagglutinin (HA) and PB2. In addition, the adapted viruses exhibited a reduced ability to react with ferret antisera against A/N. In conclusion, prolonged passaging allowed influenza A/N to adapt to different hosts, as indicated by a high increase in proliferative capacity that was accompanied by an antigenic alteration leading to amino acid substitutions. Public Library of Science 2015-06-16 /pmc/articles/PMC4469301/ /pubmed/26079133 http://dx.doi.org/10.1371/journal.pone.0130208 Text en © 2015 Ainai et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ainai, Akira
Hasegawa, Hideki
Obuchi, Masatsugu
Odagiri, Takato
Ujike, Makoto
Shirakura, Masayuki
Nobusawa, Eri
Tashiro, Masato
Asanuma, Hideki
Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan
title Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan
title_full Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan
title_fullStr Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan
title_full_unstemmed Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan
title_short Host Adaptation and the Alteration of Viral Properties of the First Influenza A/H1N1pdm09 Virus Isolated in Japan
title_sort host adaptation and the alteration of viral properties of the first influenza a/h1n1pdm09 virus isolated in japan
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4469301/
https://www.ncbi.nlm.nih.gov/pubmed/26079133
http://dx.doi.org/10.1371/journal.pone.0130208
work_keys_str_mv AT ainaiakira hostadaptationandthealterationofviralpropertiesofthefirstinfluenzaah1n1pdm09virusisolatedinjapan
AT hasegawahideki hostadaptationandthealterationofviralpropertiesofthefirstinfluenzaah1n1pdm09virusisolatedinjapan
AT obuchimasatsugu hostadaptationandthealterationofviralpropertiesofthefirstinfluenzaah1n1pdm09virusisolatedinjapan
AT odagiritakato hostadaptationandthealterationofviralpropertiesofthefirstinfluenzaah1n1pdm09virusisolatedinjapan
AT ujikemakoto hostadaptationandthealterationofviralpropertiesofthefirstinfluenzaah1n1pdm09virusisolatedinjapan
AT shirakuramasayuki hostadaptationandthealterationofviralpropertiesofthefirstinfluenzaah1n1pdm09virusisolatedinjapan
AT nobusawaeri hostadaptationandthealterationofviralpropertiesofthefirstinfluenzaah1n1pdm09virusisolatedinjapan
AT tashiromasato hostadaptationandthealterationofviralpropertiesofthefirstinfluenzaah1n1pdm09virusisolatedinjapan
AT asanumahideki hostadaptationandthealterationofviralpropertiesofthefirstinfluenzaah1n1pdm09virusisolatedinjapan