An optimized fluorescent probe for visualizing glutamate neurotransmission

We describe an intensity-based glutamate-sensing fluorescent reporter (“iGluSnFR”) with signal-to-noise ratio and kinetics appropriate for in vivo imaging. We engineered iGluSnFR in vitro to maximize its fluorescence change, and validated its utility for visualizing glutamate release by neurons and...

Descripción completa

Detalles Bibliográficos
Autores principales: Marvin, Jonathan S., Borghuis, Bart G., Tian, Lin, Cichon, Joseph, Harnett, Mark T., Akerboom, Jasper, Gordus, Andrew, Renninger, Sabine L., Chen, Tsai-Wen, Bargmann, Cornelia I., Orger, Michael B., Schreiter, Eric R., Demb, Jonathan B., Gan, Wen-Biao, Hires, S. Andrew, Looger, Loren L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4469972/
https://www.ncbi.nlm.nih.gov/pubmed/23314171
http://dx.doi.org/10.1038/nmeth.2333

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4469972/
https://www.ncbi.nlm.nih.gov/pubmed/23314171
http://dx.doi.org/10.1038/nmeth.2333

Ejemplares similares