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A cell cycle-controlled redox switch regulates the topoisomerase IV activity

Topoisomerase IV (topo IV), an essential factor during chromosome segregation, resolves the catenated chromosomes at the end of each replication cycle. How the decatenating activity of the topo IV is regulated during the early stages of the chromosome cycle despite being in continuous association wi...

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Autores principales: Narayanan, Sharath, Janakiraman, Balaganesh, Kumar, Lokesh, Radhakrishnan, Sunish Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4470285/
https://www.ncbi.nlm.nih.gov/pubmed/26063575
http://dx.doi.org/10.1101/gad.257030.114
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author Narayanan, Sharath
Janakiraman, Balaganesh
Kumar, Lokesh
Radhakrishnan, Sunish Kumar
author_facet Narayanan, Sharath
Janakiraman, Balaganesh
Kumar, Lokesh
Radhakrishnan, Sunish Kumar
author_sort Narayanan, Sharath
collection PubMed
description Topoisomerase IV (topo IV), an essential factor during chromosome segregation, resolves the catenated chromosomes at the end of each replication cycle. How the decatenating activity of the topo IV is regulated during the early stages of the chromosome cycle despite being in continuous association with the chromosome remains poorly understood. Here we report a novel cell cycle-regulated protein in Caulobacter crescentus, NstA (negative switch for topo IV decatenation activity), that inhibits the decatenation activity of the topo IV during early stages of the cell cycle. We demonstrate that in C. crescentus, NstA acts by binding to the ParC DNA-binding subunit of topo IV. Most importantly, we uncover a dynamic oscillation of the intracellular redox state during the cell cycle, which correlates with and controls NstA activity. Thus, we propose that predetermined dynamic intracellular redox fluctuations may act as a global regulatory switch to control cellular development and cell cycle progression and may help retain pathogens in a suitable cell cycle state when encountering redox stress from the host immune response.
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spelling pubmed-44702852015-06-19 A cell cycle-controlled redox switch regulates the topoisomerase IV activity Narayanan, Sharath Janakiraman, Balaganesh Kumar, Lokesh Radhakrishnan, Sunish Kumar Genes Dev Research Paper Topoisomerase IV (topo IV), an essential factor during chromosome segregation, resolves the catenated chromosomes at the end of each replication cycle. How the decatenating activity of the topo IV is regulated during the early stages of the chromosome cycle despite being in continuous association with the chromosome remains poorly understood. Here we report a novel cell cycle-regulated protein in Caulobacter crescentus, NstA (negative switch for topo IV decatenation activity), that inhibits the decatenation activity of the topo IV during early stages of the cell cycle. We demonstrate that in C. crescentus, NstA acts by binding to the ParC DNA-binding subunit of topo IV. Most importantly, we uncover a dynamic oscillation of the intracellular redox state during the cell cycle, which correlates with and controls NstA activity. Thus, we propose that predetermined dynamic intracellular redox fluctuations may act as a global regulatory switch to control cellular development and cell cycle progression and may help retain pathogens in a suitable cell cycle state when encountering redox stress from the host immune response. Cold Spring Harbor Laboratory Press 2015-06-01 /pmc/articles/PMC4470285/ /pubmed/26063575 http://dx.doi.org/10.1101/gad.257030.114 Text en © 2015 Narayanan et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by/4.0/ This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Paper
Narayanan, Sharath
Janakiraman, Balaganesh
Kumar, Lokesh
Radhakrishnan, Sunish Kumar
A cell cycle-controlled redox switch regulates the topoisomerase IV activity
title A cell cycle-controlled redox switch regulates the topoisomerase IV activity
title_full A cell cycle-controlled redox switch regulates the topoisomerase IV activity
title_fullStr A cell cycle-controlled redox switch regulates the topoisomerase IV activity
title_full_unstemmed A cell cycle-controlled redox switch regulates the topoisomerase IV activity
title_short A cell cycle-controlled redox switch regulates the topoisomerase IV activity
title_sort cell cycle-controlled redox switch regulates the topoisomerase iv activity
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4470285/
https://www.ncbi.nlm.nih.gov/pubmed/26063575
http://dx.doi.org/10.1101/gad.257030.114
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