Cargando…
A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells
The development of new gene-editing tools, in particular the CRISPR/Cas system, has greatly facilitated site-specific mutagenesis in human embryonic stem cells (hESCs), including the introduction or correction of patient-specific mutations for disease modeling. However, integration of a reporter gen...
| Autores principales: | , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2015
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471821/ https://www.ncbi.nlm.nih.gov/pubmed/26028531 http://dx.doi.org/10.1016/j.stemcr.2015.04.016 |