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Live-cell multiphoton fluorescence correlation spectroscopy with an improved large Stokes shift fluorescent protein
We report an improved variant of mKeima, a monomeric long Stokes shift red fluorescent protein, hmKeima8.5. The increased intracellular brightness and large Stokes shift (∼180 nm) make it an excellent partner with teal fluorescent protein (mTFP1) for multiphoton, multicolor applications. Excitation...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4472016/ https://www.ncbi.nlm.nih.gov/pubmed/25877871 http://dx.doi.org/10.1091/mbc.E14-10-1473 |