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Modification of comet-FISH technique by using temperature instead of chemical denaturation

Comet-FISH technique is an extension of commonly used comet assay. Its purpose is to determine whether primary DNA damage which comet assay detects occurred within a sequence of interest that is visualized by hybridization of fluorescent probe. Presence of the signal in comet tail indicates impaired...

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Detalles Bibliográficos
Autores principales: Mladinic, Marin, Zeljezic, Davor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4472953/
https://www.ncbi.nlm.nih.gov/pubmed/26150949
http://dx.doi.org/10.1016/j.mex.2014.08.010