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GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice

Microinjection is considered the gold standard technique for delivery of nucleic acids (NAs; transgenes or genome editing tools such as CRISPR/Cas9 systems) into embryos, for creating genetically modified organisms. It requires sophisticated equipment as wel as well-trained and highly skilled person...

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Detalles Bibliográficos
Autores principales: Takahashi, Gou, Gurumurthy, Channabasavaiah B, Wada, Kenta, Miura, Hiromi, Sato, Masahiro, Ohtsuka, Masato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476150/
https://www.ncbi.nlm.nih.gov/pubmed/26096991
http://dx.doi.org/10.1038/srep11406