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Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III

Human RNase P is implicated in transcription of small non-coding RNA genes by RNA polymerase III (Pol III), but the precise role of this ribonucleoprotein therein remains unknown. We here show that targeted destruction of HeLa nuclear RNase P inhibits transcription of 5S rRNA genes in whole cell ext...

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Autores principales: Serruya, Raphael, Orlovetskie, Natalie, Reiner, Robert, Dehtiar-Zilber, Yana, Wesolowski, Donna, Altman, Sidney, Jarrous, Nayef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477669/
https://www.ncbi.nlm.nih.gov/pubmed/25953854
http://dx.doi.org/10.1093/nar/gkv447
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author Serruya, Raphael
Orlovetskie, Natalie
Reiner, Robert
Dehtiar-Zilber, Yana
Wesolowski, Donna
Altman, Sidney
Jarrous, Nayef
author_facet Serruya, Raphael
Orlovetskie, Natalie
Reiner, Robert
Dehtiar-Zilber, Yana
Wesolowski, Donna
Altman, Sidney
Jarrous, Nayef
author_sort Serruya, Raphael
collection PubMed
description Human RNase P is implicated in transcription of small non-coding RNA genes by RNA polymerase III (Pol III), but the precise role of this ribonucleoprotein therein remains unknown. We here show that targeted destruction of HeLa nuclear RNase P inhibits transcription of 5S rRNA genes in whole cell extracts, if this precedes the stage of initiation complex formation. Biochemical purification analyses further reveal that this ribonucleoprotein is recruited to 5S rRNA genes as a part of proficient initiation complexes and the activity persists at reinitiation. Knockdown of RNase P abolishes the assembly of initiation complexes by preventing the formation of the initiation sub-complex of Pol III. Our results demonstrate that the structural intactness, but not the endoribonucleolytic activity per se, of RNase P is critical for the function of Pol III in cells and in extracts.
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spelling pubmed-44776692015-06-29 Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III Serruya, Raphael Orlovetskie, Natalie Reiner, Robert Dehtiar-Zilber, Yana Wesolowski, Donna Altman, Sidney Jarrous, Nayef Nucleic Acids Res Gene regulation, Chromatin and Epigenetics Human RNase P is implicated in transcription of small non-coding RNA genes by RNA polymerase III (Pol III), but the precise role of this ribonucleoprotein therein remains unknown. We here show that targeted destruction of HeLa nuclear RNase P inhibits transcription of 5S rRNA genes in whole cell extracts, if this precedes the stage of initiation complex formation. Biochemical purification analyses further reveal that this ribonucleoprotein is recruited to 5S rRNA genes as a part of proficient initiation complexes and the activity persists at reinitiation. Knockdown of RNase P abolishes the assembly of initiation complexes by preventing the formation of the initiation sub-complex of Pol III. Our results demonstrate that the structural intactness, but not the endoribonucleolytic activity per se, of RNase P is critical for the function of Pol III in cells and in extracts. Oxford University Press 2015-06-23 2015-05-07 /pmc/articles/PMC4477669/ /pubmed/25953854 http://dx.doi.org/10.1093/nar/gkv447 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Gene regulation, Chromatin and Epigenetics
Serruya, Raphael
Orlovetskie, Natalie
Reiner, Robert
Dehtiar-Zilber, Yana
Wesolowski, Donna
Altman, Sidney
Jarrous, Nayef
Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III
title Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III
title_full Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III
title_fullStr Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III
title_full_unstemmed Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III
title_short Human RNase P ribonucleoprotein is required for formation of initiation complexes of RNA polymerase III
title_sort human rnase p ribonucleoprotein is required for formation of initiation complexes of rna polymerase iii
topic Gene regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477669/
https://www.ncbi.nlm.nih.gov/pubmed/25953854
http://dx.doi.org/10.1093/nar/gkv447
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