Cargando…

A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance

Centromeres are defined by the presence of CENP-A nucleosomes in chromatin and are essential for accurate chromosome segregation. Centromeric chromatin epigenetically seeds new CENP-A nucleosome formation, thereby maintaining functional centromeres as cells divide. The features within centromeric ch...

Descripción completa

Detalles Bibliográficos
Autores principales: Westhorpe, Frederick G., Fuller, Colin J., Straight, Aaron F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477859/
https://www.ncbi.nlm.nih.gov/pubmed/26076692
http://dx.doi.org/10.1083/jcb.201503132
_version_ 1782377817725468672
author Westhorpe, Frederick G.
Fuller, Colin J.
Straight, Aaron F.
author_facet Westhorpe, Frederick G.
Fuller, Colin J.
Straight, Aaron F.
author_sort Westhorpe, Frederick G.
collection PubMed
description Centromeres are defined by the presence of CENP-A nucleosomes in chromatin and are essential for accurate chromosome segregation. Centromeric chromatin epigenetically seeds new CENP-A nucleosome formation, thereby maintaining functional centromeres as cells divide. The features within centromeric chromatin that direct new CENP-A assembly remain unclear. Here, we developed a cell-free CENP-A assembly system that enabled the study of chromatin-bound CENP-A and soluble CENP-A separately. We show that two distinct domains of CENP-A within existing CENP-A nucleosomes are required for new CENP-A assembly and that CENP-A nucleosomes recruit the CENP-A assembly factors CENP-C and M18BP1 independently. Furthermore, we demonstrate that the mechanism of CENP-C recruitment to centromeres is dependent on the density of underlying CENP-A nucleosomes.
format Online
Article
Text
id pubmed-4477859
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-44778592015-12-22 A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance Westhorpe, Frederick G. Fuller, Colin J. Straight, Aaron F. J Cell Biol Research Articles Centromeres are defined by the presence of CENP-A nucleosomes in chromatin and are essential for accurate chromosome segregation. Centromeric chromatin epigenetically seeds new CENP-A nucleosome formation, thereby maintaining functional centromeres as cells divide. The features within centromeric chromatin that direct new CENP-A assembly remain unclear. Here, we developed a cell-free CENP-A assembly system that enabled the study of chromatin-bound CENP-A and soluble CENP-A separately. We show that two distinct domains of CENP-A within existing CENP-A nucleosomes are required for new CENP-A assembly and that CENP-A nucleosomes recruit the CENP-A assembly factors CENP-C and M18BP1 independently. Furthermore, we demonstrate that the mechanism of CENP-C recruitment to centromeres is dependent on the density of underlying CENP-A nucleosomes. The Rockefeller University Press 2015-06-22 /pmc/articles/PMC4477859/ /pubmed/26076692 http://dx.doi.org/10.1083/jcb.201503132 Text en © 2015 Westhorpe et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Westhorpe, Frederick G.
Fuller, Colin J.
Straight, Aaron F.
A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance
title A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance
title_full A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance
title_fullStr A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance
title_full_unstemmed A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance
title_short A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance
title_sort cell-free cenp-a assembly system defines the chromatin requirements for centromere maintenance
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477859/
https://www.ncbi.nlm.nih.gov/pubmed/26076692
http://dx.doi.org/10.1083/jcb.201503132
work_keys_str_mv AT westhorpefrederickg acellfreecenpaassemblysystemdefinesthechromatinrequirementsforcentromeremaintenance
AT fullercolinj acellfreecenpaassemblysystemdefinesthechromatinrequirementsforcentromeremaintenance
AT straightaaronf acellfreecenpaassemblysystemdefinesthechromatinrequirementsforcentromeremaintenance
AT westhorpefrederickg cellfreecenpaassemblysystemdefinesthechromatinrequirementsforcentromeremaintenance
AT fullercolinj cellfreecenpaassemblysystemdefinesthechromatinrequirementsforcentromeremaintenance
AT straightaaronf cellfreecenpaassemblysystemdefinesthechromatinrequirementsforcentromeremaintenance