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A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance
Centromeres are defined by the presence of CENP-A nucleosomes in chromatin and are essential for accurate chromosome segregation. Centromeric chromatin epigenetically seeds new CENP-A nucleosome formation, thereby maintaining functional centromeres as cells divide. The features within centromeric ch...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477859/ https://www.ncbi.nlm.nih.gov/pubmed/26076692 http://dx.doi.org/10.1083/jcb.201503132 |
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author | Westhorpe, Frederick G. Fuller, Colin J. Straight, Aaron F. |
author_facet | Westhorpe, Frederick G. Fuller, Colin J. Straight, Aaron F. |
author_sort | Westhorpe, Frederick G. |
collection | PubMed |
description | Centromeres are defined by the presence of CENP-A nucleosomes in chromatin and are essential for accurate chromosome segregation. Centromeric chromatin epigenetically seeds new CENP-A nucleosome formation, thereby maintaining functional centromeres as cells divide. The features within centromeric chromatin that direct new CENP-A assembly remain unclear. Here, we developed a cell-free CENP-A assembly system that enabled the study of chromatin-bound CENP-A and soluble CENP-A separately. We show that two distinct domains of CENP-A within existing CENP-A nucleosomes are required for new CENP-A assembly and that CENP-A nucleosomes recruit the CENP-A assembly factors CENP-C and M18BP1 independently. Furthermore, we demonstrate that the mechanism of CENP-C recruitment to centromeres is dependent on the density of underlying CENP-A nucleosomes. |
format | Online Article Text |
id | pubmed-4477859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-44778592015-12-22 A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance Westhorpe, Frederick G. Fuller, Colin J. Straight, Aaron F. J Cell Biol Research Articles Centromeres are defined by the presence of CENP-A nucleosomes in chromatin and are essential for accurate chromosome segregation. Centromeric chromatin epigenetically seeds new CENP-A nucleosome formation, thereby maintaining functional centromeres as cells divide. The features within centromeric chromatin that direct new CENP-A assembly remain unclear. Here, we developed a cell-free CENP-A assembly system that enabled the study of chromatin-bound CENP-A and soluble CENP-A separately. We show that two distinct domains of CENP-A within existing CENP-A nucleosomes are required for new CENP-A assembly and that CENP-A nucleosomes recruit the CENP-A assembly factors CENP-C and M18BP1 independently. Furthermore, we demonstrate that the mechanism of CENP-C recruitment to centromeres is dependent on the density of underlying CENP-A nucleosomes. The Rockefeller University Press 2015-06-22 /pmc/articles/PMC4477859/ /pubmed/26076692 http://dx.doi.org/10.1083/jcb.201503132 Text en © 2015 Westhorpe et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Westhorpe, Frederick G. Fuller, Colin J. Straight, Aaron F. A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance |
title | A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance |
title_full | A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance |
title_fullStr | A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance |
title_full_unstemmed | A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance |
title_short | A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance |
title_sort | cell-free cenp-a assembly system defines the chromatin requirements for centromere maintenance |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4477859/ https://www.ncbi.nlm.nih.gov/pubmed/26076692 http://dx.doi.org/10.1083/jcb.201503132 |
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