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16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice

BACKGROUND: Characterisation of the bacterial composition of the gut microbiota is increasingly carried out with a view to establish the role of different bacterial species in causation or prevention of disease. It is thus essential that the methods used to determine the microbial composition are ro...

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Detalles Bibliográficos
Autores principales: Walker, Alan W., Martin, Jennifer C., Scott, Paul, Parkhill, Julian, Flint, Harry J., Scott, Karen P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4482049/
https://www.ncbi.nlm.nih.gov/pubmed/26120470
http://dx.doi.org/10.1186/s40168-015-0087-4