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16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice
BACKGROUND: Characterisation of the bacterial composition of the gut microbiota is increasingly carried out with a view to establish the role of different bacterial species in causation or prevention of disease. It is thus essential that the methods used to determine the microbial composition are ro...
Autores principales: | Walker, Alan W., Martin, Jennifer C., Scott, Paul, Parkhill, Julian, Flint, Harry J., Scott, Karen P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4482049/ https://www.ncbi.nlm.nih.gov/pubmed/26120470 http://dx.doi.org/10.1186/s40168-015-0087-4 |
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