Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture

Type-I interferons (IFNs) induce the expression of hundreds of cellular genes, some of which have direct antiviral activities. Although IFNs restrict different steps of HIV replication cycle, their dominant antiviral effect remains unclear. We first quantified the inhibition of HIV replication by IF...

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Detalles Bibliográficos
Autores principales: Ikeda, Hiroki, Godinho-Santos, Ana, Rato, Sylvie, Vanwalscappel, Bénédicte, Clavel, François, Aihara, Kazuyuki, Iwami, Shingo, Mammano, Fabrizio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4483772/
https://www.ncbi.nlm.nih.gov/pubmed/26119462
http://dx.doi.org/10.1038/srep11761
Descripción
Sumario:Type-I interferons (IFNs) induce the expression of hundreds of cellular genes, some of which have direct antiviral activities. Although IFNs restrict different steps of HIV replication cycle, their dominant antiviral effect remains unclear. We first quantified the inhibition of HIV replication by IFN in tissue culture, using viruses with different tropism and growth kinetics. By combining experimental and mathematical analyses, we determined quantitative estimates for key parameters of HIV replication and inhibition, and demonstrate that IFN mainly inhibits de novo infection (33% and 47% for a X4- and a R5-strain, respectively), rather than virus production (15% and 6% for the X4 and R5 strains, respectively). This finding is in agreement with patient-derived data analyses.

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