Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture

Type-I interferons (IFNs) induce the expression of hundreds of cellular genes, some of which have direct antiviral activities. Although IFNs restrict different steps of HIV replication cycle, their dominant antiviral effect remains unclear. We first quantified the inhibition of HIV replication by IF...

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Autores principales: Ikeda, Hiroki, Godinho-Santos, Ana, Rato, Sylvie, Vanwalscappel, Bénédicte, Clavel, François, Aihara, Kazuyuki, Iwami, Shingo, Mammano, Fabrizio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4483772/
https://www.ncbi.nlm.nih.gov/pubmed/26119462
http://dx.doi.org/10.1038/srep11761
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author Ikeda, Hiroki
Godinho-Santos, Ana
Rato, Sylvie
Vanwalscappel, Bénédicte
Clavel, François
Aihara, Kazuyuki
Iwami, Shingo
Mammano, Fabrizio
author_facet Ikeda, Hiroki
Godinho-Santos, Ana
Rato, Sylvie
Vanwalscappel, Bénédicte
Clavel, François
Aihara, Kazuyuki
Iwami, Shingo
Mammano, Fabrizio
author_sort Ikeda, Hiroki
collection PubMed
description Type-I interferons (IFNs) induce the expression of hundreds of cellular genes, some of which have direct antiviral activities. Although IFNs restrict different steps of HIV replication cycle, their dominant antiviral effect remains unclear. We first quantified the inhibition of HIV replication by IFN in tissue culture, using viruses with different tropism and growth kinetics. By combining experimental and mathematical analyses, we determined quantitative estimates for key parameters of HIV replication and inhibition, and demonstrate that IFN mainly inhibits de novo infection (33% and 47% for a X4- and a R5-strain, respectively), rather than virus production (15% and 6% for the X4 and R5 strains, respectively). This finding is in agreement with patient-derived data analyses.
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spelling pubmed-44837722015-07-08 Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture Ikeda, Hiroki Godinho-Santos, Ana Rato, Sylvie Vanwalscappel, Bénédicte Clavel, François Aihara, Kazuyuki Iwami, Shingo Mammano, Fabrizio Sci Rep Article Type-I interferons (IFNs) induce the expression of hundreds of cellular genes, some of which have direct antiviral activities. Although IFNs restrict different steps of HIV replication cycle, their dominant antiviral effect remains unclear. We first quantified the inhibition of HIV replication by IFN in tissue culture, using viruses with different tropism and growth kinetics. By combining experimental and mathematical analyses, we determined quantitative estimates for key parameters of HIV replication and inhibition, and demonstrate that IFN mainly inhibits de novo infection (33% and 47% for a X4- and a R5-strain, respectively), rather than virus production (15% and 6% for the X4 and R5 strains, respectively). This finding is in agreement with patient-derived data analyses. Nature Publishing Group 2015-06-29 /pmc/articles/PMC4483772/ /pubmed/26119462 http://dx.doi.org/10.1038/srep11761 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ikeda, Hiroki
Godinho-Santos, Ana
Rato, Sylvie
Vanwalscappel, Bénédicte
Clavel, François
Aihara, Kazuyuki
Iwami, Shingo
Mammano, Fabrizio
Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture
title Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture
title_full Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture
title_fullStr Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture
title_full_unstemmed Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture
title_short Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture
title_sort quantifying the antiviral effect of ifn on hiv-1 replication in cell culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4483772/
https://www.ncbi.nlm.nih.gov/pubmed/26119462
http://dx.doi.org/10.1038/srep11761
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