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Optimizing fluorescent protein trios for 3-Way FRET imaging of protein interactions in living cells
Powerful new methods have extended FRET microscopy to the imaging of three or more interacting proteins inside living cells. Here, we compared widely available fluorescent proteins to find the best trio for 3-Way FRET imaging. We focused on readily available cyan, yellow, and red proteins that have...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4487001/ https://www.ncbi.nlm.nih.gov/pubmed/26130463 http://dx.doi.org/10.1038/srep10270 |