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Combining Random Gene Fission and Rational Gene Fusion To Discover Near-Infrared Fluorescent Protein Fragments That Report on Protein–Protein Interactions

[Image: see text] Gene fission can convert monomeric proteins into two-piece catalysts, reporters, and transcription factors for systems and synthetic biology. However, some proteins can be challenging to fragment without disrupting function, such as near-infrared fluorescent protein (IFP). We descr...

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Detalles Bibliográficos
Autores principales: Pandey, Naresh, Nobles, Christopher L., Zechiedrich, Lynn, Maresso, Anthony W., Silberg, Jonathan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4487222/
https://www.ncbi.nlm.nih.gov/pubmed/25265085
http://dx.doi.org/10.1021/sb5002938