Cargando…
A simple and effective method to encapsulate tobacco mesophyll protoplasts to maintain cell viability()
Protoplasts have been widely used for genetic transformation, cell fusion, and somatic mutation due to the absence of a cell wall. However, without the protection of a cell wall, protoplasts are easy to rupture and aggregate during washing, collecting, and gene transfection. In this work, we propose...
| Autores principales: | , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2014
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4487327/ https://www.ncbi.nlm.nih.gov/pubmed/26150968 http://dx.doi.org/10.1016/j.mex.2014.11.004 |