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Modification of a commercial DNA extraction kit for safe and rapid recovery of DNA and RNA simultaneously from soil, without the use of harmful solvents

An optimized method, based on the coupling of two commercial kits, is described for the extraction of soil nucleic acids, with simultaneous extraction and purification of DNA and RNA following a cascade scheme and avoiding the use of harmful solvents. The protocol canmonitor the variations in the re...

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Detalles Bibliográficos
Autores principales: Tournier, E., Amenc, L., Pablo, A.L., Legname, E., Blanchart, E., Plassard, C., Robin, A., Bernard, L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4487712/
https://www.ncbi.nlm.nih.gov/pubmed/26150987
http://dx.doi.org/10.1016/j.mex.2015.03.007