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An improved 96-well turbidity assay for T4 lysozyme activity

T4 lysozyme (T4L) is an important model system for investigating the relationship between protein structure and function. Despite being extensively studied, a reliable, quantitative activity assay for T4L has not been developed. Here, we present an improved T4L turbidity assay as well as an affinity...

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Detalles Bibliográficos
Autores principales: Toro, Tasha B., Nguyen, Thao P., Watt, Terry J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4487725/
https://www.ncbi.nlm.nih.gov/pubmed/26150996
http://dx.doi.org/10.1016/j.mex.2015.05.004