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An Optimized Fluorescence-Based Bidimensional Immunoproteomic Approach for Accurate Screening of Autoantibodies

Serological proteome analysis (SERPA) combines classical proteomic technology with effective separation of cellular protein extracts on two-dimensional gel electrophoresis, western blotting, and identification of the antigenic spot of interest by mass spectrometry. A critical point is related to the...

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Detalles Bibliográficos
Autores principales: Dutoit-Lefèvre, Virginie, Dubucquoi, Sylvain, Launay, David, Sobanski, Vincent, Dussart, Patricia, Chafey, Philippe, Broussard, Cédric, Duban-Deweer, Sophie, Vermersch, Patrick, Prin, Lionel, Lefranc, Didier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4489013/
https://www.ncbi.nlm.nih.gov/pubmed/26132557
http://dx.doi.org/10.1371/journal.pone.0132142