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Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation

BACKGROUND: The complex microbiome of the gut has an enormous impact on human health. Analysis of the transcriptional activity of microorganisms through mRNA sequencing (metatranscriptomics) opens a completely new window into their activity in vivo, but it is highly challenging due to numerous techn...

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Detalles Bibliográficos
Autores principales: Reck, Michael, Tomasch, Jürgen, Deng, Zhiluo, Jarek, Michael, Husemann, Peter, Wagner-Döbler, Irene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490624/
https://www.ncbi.nlm.nih.gov/pubmed/26140923
http://dx.doi.org/10.1186/s12864-015-1694-y