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Grb2 monomer–dimer equilibrium determines normal versus oncogenic function
The adaptor protein growth factor receptor-bound protein 2 (Grb2) is ubiquitously expressed in eukaryotic cells and involved in a multitude of intracellular protein interactions. Grb2 plays a pivotal role in tyrosine kinase-mediated signal transduction including linking receptor tyrosine kinases to...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491180/ https://www.ncbi.nlm.nih.gov/pubmed/26103942 http://dx.doi.org/10.1038/ncomms8354 |
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author | Ahmed, Zamal Timsah, Zahra Suen, Kin M. Cook, Nathan P. Lee, Gilbert R. Lin, Chi-Chuan Gagea, Mihai Marti, Angel A. Ladbury, John E. |
author_facet | Ahmed, Zamal Timsah, Zahra Suen, Kin M. Cook, Nathan P. Lee, Gilbert R. Lin, Chi-Chuan Gagea, Mihai Marti, Angel A. Ladbury, John E. |
author_sort | Ahmed, Zamal |
collection | PubMed |
description | The adaptor protein growth factor receptor-bound protein 2 (Grb2) is ubiquitously expressed in eukaryotic cells and involved in a multitude of intracellular protein interactions. Grb2 plays a pivotal role in tyrosine kinase-mediated signal transduction including linking receptor tyrosine kinases to the Ras/mitogen-activated protein (MAP) kinase pathway, which is implicated in oncogenic outcome. Grb2 exists in a constitutive equilibrium between monomeric and dimeric states. Here we show that only monomeric Grb2 is capable of binding to SOS and upregulating MAP kinase signalling and that the dimeric state is inhibitory to this process. Phosphorylation of tyrosine 160 (Y160) on Grb2, or binding of a tyrosylphosphate-containing ligand to the SH2 domain of Grb2, results in dimer dissociation. Phosphorylation of Y160 on Grb2 is readily detectable in the malignant forms of human prostate, colon and breast cancers. The self-association/dissociation of Grb2 represents a switch that regulates MAP kinase activity and hence controls cancer progression. |
format | Online Article Text |
id | pubmed-4491180 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Pub. Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-44911802015-07-08 Grb2 monomer–dimer equilibrium determines normal versus oncogenic function Ahmed, Zamal Timsah, Zahra Suen, Kin M. Cook, Nathan P. Lee, Gilbert R. Lin, Chi-Chuan Gagea, Mihai Marti, Angel A. Ladbury, John E. Nat Commun Article The adaptor protein growth factor receptor-bound protein 2 (Grb2) is ubiquitously expressed in eukaryotic cells and involved in a multitude of intracellular protein interactions. Grb2 plays a pivotal role in tyrosine kinase-mediated signal transduction including linking receptor tyrosine kinases to the Ras/mitogen-activated protein (MAP) kinase pathway, which is implicated in oncogenic outcome. Grb2 exists in a constitutive equilibrium between monomeric and dimeric states. Here we show that only monomeric Grb2 is capable of binding to SOS and upregulating MAP kinase signalling and that the dimeric state is inhibitory to this process. Phosphorylation of tyrosine 160 (Y160) on Grb2, or binding of a tyrosylphosphate-containing ligand to the SH2 domain of Grb2, results in dimer dissociation. Phosphorylation of Y160 on Grb2 is readily detectable in the malignant forms of human prostate, colon and breast cancers. The self-association/dissociation of Grb2 represents a switch that regulates MAP kinase activity and hence controls cancer progression. Nature Pub. Group 2015-06-24 /pmc/articles/PMC4491180/ /pubmed/26103942 http://dx.doi.org/10.1038/ncomms8354 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Ahmed, Zamal Timsah, Zahra Suen, Kin M. Cook, Nathan P. Lee, Gilbert R. Lin, Chi-Chuan Gagea, Mihai Marti, Angel A. Ladbury, John E. Grb2 monomer–dimer equilibrium determines normal versus oncogenic function |
title | Grb2 monomer–dimer equilibrium determines normal versus oncogenic function |
title_full | Grb2 monomer–dimer equilibrium determines normal versus oncogenic function |
title_fullStr | Grb2 monomer–dimer equilibrium determines normal versus oncogenic function |
title_full_unstemmed | Grb2 monomer–dimer equilibrium determines normal versus oncogenic function |
title_short | Grb2 monomer–dimer equilibrium determines normal versus oncogenic function |
title_sort | grb2 monomer–dimer equilibrium determines normal versus oncogenic function |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491180/ https://www.ncbi.nlm.nih.gov/pubmed/26103942 http://dx.doi.org/10.1038/ncomms8354 |
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