Stepwise error-prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilic Bacillus sp.

Cyclodextrin glucanotransferase (EC 2.4.1.19) from the alkaliphilic Bacillus sp. G-825-6 converts starch mainly to γ-cyclodextrin (CD(8)). A combination of error-prone PCR and DNA shuffling was used to obtain variants of this enzyme with higher product specificity for CD(8) and a broad pH activity r...

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Detalles Bibliográficos
Autores principales: Melzer, Susanne, Sonnendecker, Christian, Föllner, Christina, Zimmermann, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Research article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491590/
https://www.ncbi.nlm.nih.gov/pubmed/26155461
http://dx.doi.org/10.1016/j.fob.2015.06.002

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491590/
https://www.ncbi.nlm.nih.gov/pubmed/26155461
http://dx.doi.org/10.1016/j.fob.2015.06.002

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