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Stepwise error-prone PCR and DNA shuffling changed the pH activity range and product specificity of the cyclodextrin glucanotransferase from an alkaliphilic Bacillus sp.
Cyclodextrin glucanotransferase (EC 2.4.1.19) from the alkaliphilic Bacillus sp. G-825-6 converts starch mainly to γ-cyclodextrin (CD(8)). A combination of error-prone PCR and DNA shuffling was used to obtain variants of this enzyme with higher product specificity for CD(8) and a broad pH activity r...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491590/ https://www.ncbi.nlm.nih.gov/pubmed/26155461 http://dx.doi.org/10.1016/j.fob.2015.06.002 |