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Two-photon directed evolution of green fluorescent proteins

Directed evolution has been used extensively to improve the properties of a variety of fluorescent proteins (FPs). Evolutionary strategies, however, have not yet been used to improve the two-photon absorption (2PA) properties of a fluorescent protein, properties that are important for two-photon ima...

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Detalles Bibliográficos
Autores principales: Stoltzfus, Caleb R., Barnett, Lauren M., Drobizhev, Mikhail, Wicks, Geoffrey, Mikhaylov, Alexander, Hughes, Thomas E., Rebane, Aleksander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491718/
https://www.ncbi.nlm.nih.gov/pubmed/26145791
http://dx.doi.org/10.1038/srep11968