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Two-photon directed evolution of green fluorescent proteins
Directed evolution has been used extensively to improve the properties of a variety of fluorescent proteins (FPs). Evolutionary strategies, however, have not yet been used to improve the two-photon absorption (2PA) properties of a fluorescent protein, properties that are important for two-photon ima...
Autores principales: | Stoltzfus, Caleb R., Barnett, Lauren M., Drobizhev, Mikhail, Wicks, Geoffrey, Mikhaylov, Alexander, Hughes, Thomas E., Rebane, Aleksander |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491718/ https://www.ncbi.nlm.nih.gov/pubmed/26145791 http://dx.doi.org/10.1038/srep11968 |
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