Cargando…

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system is an adaptive immune system in bacteria and archaea that has recently been exploited for genome engineering. Mutant mice can be generated in one step through direct delivery of the CRISPR/Ca...

Descripción completa

Detalles Bibliográficos
Autores principales:	Qin, Wenning, Dion, Stephanie L., Kutny, Peter M., Zhang, Yingfan, Cheng, Albert W., Jillette, Nathaniel L., Malhotra, Ankit, Geurts, Aron M., Chen, Yi-Guang, Wang, Haoyi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Genetics Society of America 2015
Materias:	Communications
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4492369/ https://www.ncbi.nlm.nih.gov/pubmed/25819794 http://dx.doi.org/10.1534/genetics.115.176594

Ejemplares similares

An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes
por: Tröder, Simon E., et al.
Publicado: (2018)

Electroporation-Mediated Genome Editing of Livestock Zygotes
por: Lin, Jason C., et al.
Publicado: (2021)

Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing
por: Hashimoto, Masakazu, et al.
Publicado: (2015)

Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation
por: Remy, Séverine, et al.
Publicado: (2017)

Application of CRISPR/Cas9 Nuclease in Amphioxus Genome Editing
por: Su, Liuru, et al.
Publicado: (2020)

Erratum: Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing
por: Hashimoto, Masakazu, et al.
Publicado: (2015)

One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis
por: Tanihara, Fuminori, et al.
Publicado: (2021)

The cell cycle stage of bovine zygotes electroporated with CRISPR/Cas9-RNP affects frequency of Loss-of-heterozygosity editing events
por: Miskel, Dennis, et al.
Publicado: (2022)

CRISPR–Cas9-mediated genome editing and guide RNA design
por: Wiles, Michael V., et al.
Publicado: (2015)

Validation of gene editing efficiency with CRISPR-Cas9 system directly in rat zygotes using electroporation mediated delivery and embryo culture
por: Challa, Anil K., et al.
Publicado: (2021)

CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes
por: Liang, Puping, et al.
Publicado: (2015)

Author Correction: The cell cycle stage of bovine zygotes electroporated with CRISPR/Cas9-RNP affects frequency of Loss-of-heterozygosity editing events
por: Miskel, Dennis, et al.
Publicado: (2022)

Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN
por: Tanihara, Fuminori, et al.
Publicado: (2023)

One-Step Homology Mediated CRISPR-Cas Editing in Zygotes for Generating Genome Edited Cattle
por: Park, Ki-Eun, et al.
Publicado: (2020)

CRISPR/Cas-Dependent and Nuclease-Free In Vivo Therapeutic Gene Editing
por: Dasgupta, Ishani, et al.
Publicado: (2021)

Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes
por: Tanihara, Fuminori, et al.
Publicado: (2020)

Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling
por: Cheng, Albert W, et al.
Publicado: (2016)

Synergistic engineering of CRISPR-Cas nucleases enables robust mammalian genome editing
por: Chen, Yangcan, et al.
Publicado: (2022)

Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing
por: Teixeira, Marie, et al.
Publicado: (2018)

Highly Efficient Genome Editing in Plant Protoplasts by Ribonucleoprotein Delivery of CRISPR-Cas12a Nucleases
por: Zhang, Yingxiao, et al.
Publicado: (2022)

Chromosome engineering in zygotes with CRISPR/Cas9
por: Boroviak, Katharina, et al.
Publicado: (2016)

Zona pellucida treatment before CRISPR/Cas9‐mediated genome editing of porcine zygotes
por: Namula, Zhao, et al.
Publicado: (2021)

Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique
por: Xiong, Bin, et al.
Publicado: (2018)

Homology-based repair induced by CRISPR-Cas nucleases in mammalian embryo genome editing
por: Zhang, Xiya, et al.
Publicado: (2021)

Author Correction: Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing
por: Teixeira, Marie, et al.
Publicado: (2018)

Introducing gene deletions by mouse zygote electroporation of Cas12a/Cpf1
por: Dumeau, Charles-Etienne, et al.
Publicado: (2019)

Production of genome-edited mice by visualization of nucleases introduced into the embryos using electroporation
por: WAKE, Yui, et al.
Publicado: (2020)

Electroporation and genetic supply of Cas9 increase the generation efficiency of CRISPR/Cas9 knock-in alleles in C57BL/6J mouse zygotes
por: Alghadban, Samy, et al.
Publicado: (2020)

CRISPR clear? Dimeric Cas9-Fok1 nucleases improve genome-editing specificity
por: Zhang, Hongmei, et al.
Publicado: (2014)

Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering
por: Huang, Xiaoqiang, et al.
Publicado: (2022)

Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation
por: Tanihara, Fuminori, et al.
Publicado: (2018)

Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events
por: Lin, Qingyi, et al.
Publicado: (2021)

A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing
por: Wang, Yao, et al.
Publicado: (2023)

Targeted gene knock-in by CRISPR/Cas ribonucleoproteins in porcine zygotes
por: Park, Ki-Eun, et al.
Publicado: (2017)

Applications of Alternative Nucleases in the Age of CRISPR/Cas9
por: Guha, Tuhin K., et al.
Publicado: (2017)

GUIDEseq: a bioconductor package to analyze GUIDE-Seq datasets for CRISPR-Cas nucleases
por: Zhu, Lihua Julie, et al.
Publicado: (2017)

Electroporation-mediated genome editing in vitrified/warmed mouse zygotes created by IVF via ultra-superovulation
por: Nakagawa, Yoshiko, et al.
Publicado: (2018)

Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System
por: Khalil, Karim, et al.
Publicado: (2017)

In vivo Editing of the Human Mutant Rhodopsin Gene by Electroporation of Plasmid-based CRISPR/Cas9 in the Mouse Retina
por: Latella, Maria Carmela, et al.
Publicado: (2016)

TIE: A Method to Electroporate Long DNA Templates into Preimplantation Embryos for CRISPR-Cas9 Gene Editing
por: Bagheri, Hooman, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_266ngmkr18ld6dam8d9dpiis7f