Cargando…

Curcumin Ingestion Inhibits Mastocytosis and Suppresses Intestinal Anaphylaxis in a Murine Model of Food Allergy

IgE antibodies and mast cells play critical roles in the establishment of allergic responses to food antigens. Curcumin, the active ingredient of the curry spice turmeric, has anti-inflammatory properties, and thus may have the capacity to regulate Th2 cells and mucosal mast cell function during all...

Descripción completa

Detalles Bibliográficos
Autores principales: Kinney, Shannon R. M., Carlson, Logan, Ser-Dolansky, Jennifer, Thompson, Chelsea, Shah, Sagar, Gambrah, Amos, Xing, Wei, Schneider, Sallie S., Mathias, Clinton B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4493063/
https://www.ncbi.nlm.nih.gov/pubmed/26147007
http://dx.doi.org/10.1371/journal.pone.0132467
_version_ 1782379856050257920
author Kinney, Shannon R. M.
Carlson, Logan
Ser-Dolansky, Jennifer
Thompson, Chelsea
Shah, Sagar
Gambrah, Amos
Xing, Wei
Schneider, Sallie S.
Mathias, Clinton B.
author_facet Kinney, Shannon R. M.
Carlson, Logan
Ser-Dolansky, Jennifer
Thompson, Chelsea
Shah, Sagar
Gambrah, Amos
Xing, Wei
Schneider, Sallie S.
Mathias, Clinton B.
author_sort Kinney, Shannon R. M.
collection PubMed
description IgE antibodies and mast cells play critical roles in the establishment of allergic responses to food antigens. Curcumin, the active ingredient of the curry spice turmeric, has anti-inflammatory properties, and thus may have the capacity to regulate Th2 cells and mucosal mast cell function during allergic responses. We assessed whether curcumin ingestion during oral allergen exposure can modulate the development of food allergy using a murine model of ovalbumin (OVA)-induced intestinal anaphylaxis. Herein, we demonstrate that frequent ingestion of curcumin during oral OVA exposure inhibits the development of mastocytosis and intestinal anaphylaxis in OVA-challenged allergic mice. Intragastric (i.g.) exposure to OVA in sensitized BALB/c mice induced a robust IgE-mediated response accompanied by enhanced OVA-IgE levels, intestinal mastocytosis, elevated serum mMCP-1, and acute diarrhea. In contrast, mice exposed to oral curcumin throughout the experimental regimen appeared to be normal and did not exhibit intense allergic diarrhea or a significant enhancement of OVA-IgE and intestinal mast cell expansion and activation. Furthermore, allergic diarrhea, mast cell activation and expansion, and Th2 responses were also suppressed in mice exposed to curcumin during the OVA-challenge phase alone, despite the presence of elevated levels of OVA-IgE, suggesting that curcumin may have a direct suppressive effect on intestinal mast cell activation and reverse food allergy symptoms in allergen-sensitized individuals. This was confirmed by observations that curcumin attenuated the expansion of both adoptively transferred bone marrow-derived mast cells (BMMCs), and inhibited their survival and activation during cell culture. Finally, the suppression of intestinal anaphylaxis by curcumin was directly linked with the inhibition of NF-κB activation in curcumin-treated allergic mice, and curcumin inhibited the phosphorylation of the p65 subunit of NF-κB in BMMCs. In summary, our data demonstrates a protective role for curcumin during allergic responses to food antigens, suggesting that frequent ingestion of this spice may modulate the outcome of disease in susceptible individuals.
format Online
Article
Text
id pubmed-4493063
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-44930632015-07-15 Curcumin Ingestion Inhibits Mastocytosis and Suppresses Intestinal Anaphylaxis in a Murine Model of Food Allergy Kinney, Shannon R. M. Carlson, Logan Ser-Dolansky, Jennifer Thompson, Chelsea Shah, Sagar Gambrah, Amos Xing, Wei Schneider, Sallie S. Mathias, Clinton B. PLoS One Research Article IgE antibodies and mast cells play critical roles in the establishment of allergic responses to food antigens. Curcumin, the active ingredient of the curry spice turmeric, has anti-inflammatory properties, and thus may have the capacity to regulate Th2 cells and mucosal mast cell function during allergic responses. We assessed whether curcumin ingestion during oral allergen exposure can modulate the development of food allergy using a murine model of ovalbumin (OVA)-induced intestinal anaphylaxis. Herein, we demonstrate that frequent ingestion of curcumin during oral OVA exposure inhibits the development of mastocytosis and intestinal anaphylaxis in OVA-challenged allergic mice. Intragastric (i.g.) exposure to OVA in sensitized BALB/c mice induced a robust IgE-mediated response accompanied by enhanced OVA-IgE levels, intestinal mastocytosis, elevated serum mMCP-1, and acute diarrhea. In contrast, mice exposed to oral curcumin throughout the experimental regimen appeared to be normal and did not exhibit intense allergic diarrhea or a significant enhancement of OVA-IgE and intestinal mast cell expansion and activation. Furthermore, allergic diarrhea, mast cell activation and expansion, and Th2 responses were also suppressed in mice exposed to curcumin during the OVA-challenge phase alone, despite the presence of elevated levels of OVA-IgE, suggesting that curcumin may have a direct suppressive effect on intestinal mast cell activation and reverse food allergy symptoms in allergen-sensitized individuals. This was confirmed by observations that curcumin attenuated the expansion of both adoptively transferred bone marrow-derived mast cells (BMMCs), and inhibited their survival and activation during cell culture. Finally, the suppression of intestinal anaphylaxis by curcumin was directly linked with the inhibition of NF-κB activation in curcumin-treated allergic mice, and curcumin inhibited the phosphorylation of the p65 subunit of NF-κB in BMMCs. In summary, our data demonstrates a protective role for curcumin during allergic responses to food antigens, suggesting that frequent ingestion of this spice may modulate the outcome of disease in susceptible individuals. Public Library of Science 2015-07-06 /pmc/articles/PMC4493063/ /pubmed/26147007 http://dx.doi.org/10.1371/journal.pone.0132467 Text en © 2015 Kinney et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kinney, Shannon R. M.
Carlson, Logan
Ser-Dolansky, Jennifer
Thompson, Chelsea
Shah, Sagar
Gambrah, Amos
Xing, Wei
Schneider, Sallie S.
Mathias, Clinton B.
Curcumin Ingestion Inhibits Mastocytosis and Suppresses Intestinal Anaphylaxis in a Murine Model of Food Allergy
title Curcumin Ingestion Inhibits Mastocytosis and Suppresses Intestinal Anaphylaxis in a Murine Model of Food Allergy
title_full Curcumin Ingestion Inhibits Mastocytosis and Suppresses Intestinal Anaphylaxis in a Murine Model of Food Allergy
title_fullStr Curcumin Ingestion Inhibits Mastocytosis and Suppresses Intestinal Anaphylaxis in a Murine Model of Food Allergy
title_full_unstemmed Curcumin Ingestion Inhibits Mastocytosis and Suppresses Intestinal Anaphylaxis in a Murine Model of Food Allergy
title_short Curcumin Ingestion Inhibits Mastocytosis and Suppresses Intestinal Anaphylaxis in a Murine Model of Food Allergy
title_sort curcumin ingestion inhibits mastocytosis and suppresses intestinal anaphylaxis in a murine model of food allergy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4493063/
https://www.ncbi.nlm.nih.gov/pubmed/26147007
http://dx.doi.org/10.1371/journal.pone.0132467
work_keys_str_mv AT kinneyshannonrm curcuminingestioninhibitsmastocytosisandsuppressesintestinalanaphylaxisinamurinemodeloffoodallergy
AT carlsonlogan curcuminingestioninhibitsmastocytosisandsuppressesintestinalanaphylaxisinamurinemodeloffoodallergy
AT serdolanskyjennifer curcuminingestioninhibitsmastocytosisandsuppressesintestinalanaphylaxisinamurinemodeloffoodallergy
AT thompsonchelsea curcuminingestioninhibitsmastocytosisandsuppressesintestinalanaphylaxisinamurinemodeloffoodallergy
AT shahsagar curcuminingestioninhibitsmastocytosisandsuppressesintestinalanaphylaxisinamurinemodeloffoodallergy
AT gambrahamos curcuminingestioninhibitsmastocytosisandsuppressesintestinalanaphylaxisinamurinemodeloffoodallergy
AT xingwei curcuminingestioninhibitsmastocytosisandsuppressesintestinalanaphylaxisinamurinemodeloffoodallergy
AT schneidersallies curcuminingestioninhibitsmastocytosisandsuppressesintestinalanaphylaxisinamurinemodeloffoodallergy
AT mathiasclintonb curcuminingestioninhibitsmastocytosisandsuppressesintestinalanaphylaxisinamurinemodeloffoodallergy