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Addressing the link between paraoxonase-1 gene variants and the incidence of early onset myocardial infarction

INTRODUCTION: The enzyme paraoxonase-1 (PON1) represents an endogenous defense mechanism against vascular oxidative stress, thereby contributing to the prevention of atherosclerosis. Several polymorphisms have been reported in the PON1 gene, including Q192R. PON1 phenotype is commonly expressed as t...

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Autores principales: Rahman, Mohamed F. Abdel, Hashad, Ingy M., Abou-Aisha, Khaled, Abdel-Maksoud, Sahar M., Gad, Mohamed Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4495147/
https://www.ncbi.nlm.nih.gov/pubmed/26170843
http://dx.doi.org/10.5114/aoms.2015.52353
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author Rahman, Mohamed F. Abdel
Hashad, Ingy M.
Abou-Aisha, Khaled
Abdel-Maksoud, Sahar M.
Gad, Mohamed Z.
author_facet Rahman, Mohamed F. Abdel
Hashad, Ingy M.
Abou-Aisha, Khaled
Abdel-Maksoud, Sahar M.
Gad, Mohamed Z.
author_sort Rahman, Mohamed F. Abdel
collection PubMed
description INTRODUCTION: The enzyme paraoxonase-1 (PON1) represents an endogenous defense mechanism against vascular oxidative stress, thereby contributing to the prevention of atherosclerosis. Several polymorphisms have been reported in the PON1 gene, including Q192R. PON1 phenotype is commonly expressed as the paraoxonase/arylesterase ratio (PON/ARE). The major aim of this study was to investigate the association between PON1 Q192R polymorphism, PON1 phenotypes and the incidence of early-onset acute myocardial infarction (AMI) in Egyptians. MATERIAL AND METHODS: The study subjects consisted of 102 AMI patients and 72 age-matched healthy controls. Genotyping and enzyme activities were determined using PCR-RFLP and kinetic spectrophotometric assays, respectively. RESULTS: The genotype distribution for the PON1 gene was significantly different between AMI patients (QQ = 38.24%, QR = 49.02%, RR = 12.75%) and controls (QQ = 66.67%, QR = 25%, RR = 8.33%). Allele frequencies were also significantly different between patients (Q = 62.75%, R = 37.25%) and controls (Q = 79.17%, R = 20.83%). The genotypes QR and RR showed higher risk for AMI compared to the homozygous QQ (odds ratio (OR) = 3.231, p < 0.001). The average PON/ARE ratio in MI patients (1.187 ±0.1) did not differ significantly from controls (1.118 ±0.26). However, it showed a significant difference among different genotypes in both AMI patients (QQ = 0.91 ±0.11, QR = 1.09 ±0.11 and RR = 2.65 ±0.4) (p = 0.0002) and controls (QQ = 0.68 ±0.1, QR = 1.07 ±0.11 and RR = 4.89 ±2.84) (p < 0.0001). CONCLUSIONS: PON1 192R allele represents an independent risk factor for early-onset AMI in Egyptians, and PON1 Q192R polymorphism modulates the paraoxonase phenotype.
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spelling pubmed-44951472015-07-13 Addressing the link between paraoxonase-1 gene variants and the incidence of early onset myocardial infarction Rahman, Mohamed F. Abdel Hashad, Ingy M. Abou-Aisha, Khaled Abdel-Maksoud, Sahar M. Gad, Mohamed Z. Arch Med Sci Clinical Research INTRODUCTION: The enzyme paraoxonase-1 (PON1) represents an endogenous defense mechanism against vascular oxidative stress, thereby contributing to the prevention of atherosclerosis. Several polymorphisms have been reported in the PON1 gene, including Q192R. PON1 phenotype is commonly expressed as the paraoxonase/arylesterase ratio (PON/ARE). The major aim of this study was to investigate the association between PON1 Q192R polymorphism, PON1 phenotypes and the incidence of early-onset acute myocardial infarction (AMI) in Egyptians. MATERIAL AND METHODS: The study subjects consisted of 102 AMI patients and 72 age-matched healthy controls. Genotyping and enzyme activities were determined using PCR-RFLP and kinetic spectrophotometric assays, respectively. RESULTS: The genotype distribution for the PON1 gene was significantly different between AMI patients (QQ = 38.24%, QR = 49.02%, RR = 12.75%) and controls (QQ = 66.67%, QR = 25%, RR = 8.33%). Allele frequencies were also significantly different between patients (Q = 62.75%, R = 37.25%) and controls (Q = 79.17%, R = 20.83%). The genotypes QR and RR showed higher risk for AMI compared to the homozygous QQ (odds ratio (OR) = 3.231, p < 0.001). The average PON/ARE ratio in MI patients (1.187 ±0.1) did not differ significantly from controls (1.118 ±0.26). However, it showed a significant difference among different genotypes in both AMI patients (QQ = 0.91 ±0.11, QR = 1.09 ±0.11 and RR = 2.65 ±0.4) (p = 0.0002) and controls (QQ = 0.68 ±0.1, QR = 1.07 ±0.11 and RR = 4.89 ±2.84) (p < 0.0001). CONCLUSIONS: PON1 192R allele represents an independent risk factor for early-onset AMI in Egyptians, and PON1 Q192R polymorphism modulates the paraoxonase phenotype. Termedia Publishing House 2015-06-19 2015-06-19 /pmc/articles/PMC4495147/ /pubmed/26170843 http://dx.doi.org/10.5114/aoms.2015.52353 Text en Copyright © 2015 Termedia & Banach http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Research
Rahman, Mohamed F. Abdel
Hashad, Ingy M.
Abou-Aisha, Khaled
Abdel-Maksoud, Sahar M.
Gad, Mohamed Z.
Addressing the link between paraoxonase-1 gene variants and the incidence of early onset myocardial infarction
title Addressing the link between paraoxonase-1 gene variants and the incidence of early onset myocardial infarction
title_full Addressing the link between paraoxonase-1 gene variants and the incidence of early onset myocardial infarction
title_fullStr Addressing the link between paraoxonase-1 gene variants and the incidence of early onset myocardial infarction
title_full_unstemmed Addressing the link between paraoxonase-1 gene variants and the incidence of early onset myocardial infarction
title_short Addressing the link between paraoxonase-1 gene variants and the incidence of early onset myocardial infarction
title_sort addressing the link between paraoxonase-1 gene variants and the incidence of early onset myocardial infarction
topic Clinical Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4495147/
https://www.ncbi.nlm.nih.gov/pubmed/26170843
http://dx.doi.org/10.5114/aoms.2015.52353
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