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General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands

[Image: see text] Inspired by naturally occurring pH-regulated receptors, here we propose a rational approach to introduce pH-induced allostery into a wide range of DNA-based receptors. To demonstrate this we re-engineered two model DNA-based probes, a molecular beacon and a cocaine-binding aptamer,...

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Autores principales: Porchetta, Alessandro, Idili, Andrea, Vallée-Bélisle, Alexis, Ricci, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2015
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498449/
https://www.ncbi.nlm.nih.gov/pubmed/26053894
http://dx.doi.org/10.1021/acs.nanolett.5b00852
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author Porchetta, Alessandro
Idili, Andrea
Vallée-Bélisle, Alexis
Ricci, Francesco
author_facet Porchetta, Alessandro
Idili, Andrea
Vallée-Bélisle, Alexis
Ricci, Francesco
author_sort Porchetta, Alessandro
collection PubMed
description [Image: see text] Inspired by naturally occurring pH-regulated receptors, here we propose a rational approach to introduce pH-induced allostery into a wide range of DNA-based receptors. To demonstrate this we re-engineered two model DNA-based probes, a molecular beacon and a cocaine-binding aptamer, by introducing in their sequence a pH-dependent domain. We demonstrate here that we can finely tune the affinity of these model receptors and control the load/release of their specific target molecule by a simple pH change.
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spelling pubmed-44984492015-07-16 General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands Porchetta, Alessandro Idili, Andrea Vallée-Bélisle, Alexis Ricci, Francesco Nano Lett [Image: see text] Inspired by naturally occurring pH-regulated receptors, here we propose a rational approach to introduce pH-induced allostery into a wide range of DNA-based receptors. To demonstrate this we re-engineered two model DNA-based probes, a molecular beacon and a cocaine-binding aptamer, by introducing in their sequence a pH-dependent domain. We demonstrate here that we can finely tune the affinity of these model receptors and control the load/release of their specific target molecule by a simple pH change. American Chemical Society 2015-06-08 2015-07-08 /pmc/articles/PMC4498449/ /pubmed/26053894 http://dx.doi.org/10.1021/acs.nanolett.5b00852 Text en Copyright © 2015 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Porchetta, Alessandro
Idili, Andrea
Vallée-Bélisle, Alexis
Ricci, Francesco
General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands
title General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands
title_full General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands
title_fullStr General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands
title_full_unstemmed General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands
title_short General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands
title_sort general strategy to introduce ph-induced allostery in dna-based receptors to achieve controlled release of ligands
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498449/
https://www.ncbi.nlm.nih.gov/pubmed/26053894
http://dx.doi.org/10.1021/acs.nanolett.5b00852
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