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General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands
[Image: see text] Inspired by naturally occurring pH-regulated receptors, here we propose a rational approach to introduce pH-induced allostery into a wide range of DNA-based receptors. To demonstrate this we re-engineered two model DNA-based probes, a molecular beacon and a cocaine-binding aptamer,...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498449/ https://www.ncbi.nlm.nih.gov/pubmed/26053894 http://dx.doi.org/10.1021/acs.nanolett.5b00852 |
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author | Porchetta, Alessandro Idili, Andrea Vallée-Bélisle, Alexis Ricci, Francesco |
author_facet | Porchetta, Alessandro Idili, Andrea Vallée-Bélisle, Alexis Ricci, Francesco |
author_sort | Porchetta, Alessandro |
collection | PubMed |
description | [Image: see text] Inspired by naturally occurring pH-regulated receptors, here we propose a rational approach to introduce pH-induced allostery into a wide range of DNA-based receptors. To demonstrate this we re-engineered two model DNA-based probes, a molecular beacon and a cocaine-binding aptamer, by introducing in their sequence a pH-dependent domain. We demonstrate here that we can finely tune the affinity of these model receptors and control the load/release of their specific target molecule by a simple pH change. |
format | Online Article Text |
id | pubmed-4498449 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-44984492015-07-16 General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands Porchetta, Alessandro Idili, Andrea Vallée-Bélisle, Alexis Ricci, Francesco Nano Lett [Image: see text] Inspired by naturally occurring pH-regulated receptors, here we propose a rational approach to introduce pH-induced allostery into a wide range of DNA-based receptors. To demonstrate this we re-engineered two model DNA-based probes, a molecular beacon and a cocaine-binding aptamer, by introducing in their sequence a pH-dependent domain. We demonstrate here that we can finely tune the affinity of these model receptors and control the load/release of their specific target molecule by a simple pH change. American Chemical Society 2015-06-08 2015-07-08 /pmc/articles/PMC4498449/ /pubmed/26053894 http://dx.doi.org/10.1021/acs.nanolett.5b00852 Text en Copyright © 2015 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Porchetta, Alessandro Idili, Andrea Vallée-Bélisle, Alexis Ricci, Francesco General Strategy to Introduce pH-Induced Allostery in DNA-Based Receptors to Achieve Controlled Release of Ligands |
title | General Strategy to Introduce pH-Induced Allostery
in DNA-Based Receptors to Achieve Controlled Release of Ligands |
title_full | General Strategy to Introduce pH-Induced Allostery
in DNA-Based Receptors to Achieve Controlled Release of Ligands |
title_fullStr | General Strategy to Introduce pH-Induced Allostery
in DNA-Based Receptors to Achieve Controlled Release of Ligands |
title_full_unstemmed | General Strategy to Introduce pH-Induced Allostery
in DNA-Based Receptors to Achieve Controlled Release of Ligands |
title_short | General Strategy to Introduce pH-Induced Allostery
in DNA-Based Receptors to Achieve Controlled Release of Ligands |
title_sort | general strategy to introduce ph-induced allostery
in dna-based receptors to achieve controlled release of ligands |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498449/ https://www.ncbi.nlm.nih.gov/pubmed/26053894 http://dx.doi.org/10.1021/acs.nanolett.5b00852 |
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