Substitutions at the cofactor phosphate-binding site of a clostridial alcohol dehydrogenase lead to unexpected changes in substrate specificity

Changing the cofactor specificity of an enzyme from nicotinamide adenine dinucleotide 2′-phosphate (NADPH) to the more abundant NADH is a common strategy for increasing overall enzyme efficiency in microbial metabolic engineering. The aim of this study was to switch the cofactor specificity of the p...

Descripción completa

Detalles Bibliográficos
Autores principales: Maddock, Danielle J., Patrick, Wayne M., Gerth, Monica L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498498/
https://www.ncbi.nlm.nih.gov/pubmed/26034298
http://dx.doi.org/10.1093/protein/gzv028

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498498/
https://www.ncbi.nlm.nih.gov/pubmed/26034298
http://dx.doi.org/10.1093/protein/gzv028

Ejemplares similares