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Dumbbell-PCR: a method to quantify specific small RNA variants with a single nucleotide resolution at terminal sequences

Recent advances in next-generation sequencing technologies have revealed that cellular functional RNAs are not always expressed as single entities with fixed terminal sequences but as multiple isoforms bearing complex heterogeneity in both length and terminal sequences, such as isomiRs, the isoforms...

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Detalles Bibliográficos
Autores principales: Honda, Shozo, Kirino, Yohei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4499115/
https://www.ncbi.nlm.nih.gov/pubmed/25779041
http://dx.doi.org/10.1093/nar/gkv218