Nicked-site substrates for a serine recombinase reveal enzyme–DNA communications and an essential tethering role of covalent enzyme–DNA linkages

To analyse the mechanism and kinetics of DNA strand cleavages catalysed by the serine recombinase Tn3 resolvase, we made modified recombination sites with a single-strand nick in one of the two DNA strands. Resolvase acting on these sites cleaves the intact strand very rapidly, giving an abnormal ha...

Descripción completa

Detalles Bibliográficos
Autores principales: Olorunniji, Femi J., McPherson, Arlene L., Pavlou, Hania J., McIlwraith, Michael J., Brazier, John A., Cosstick, Richard, Stark, W. Marshall
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4499144/
https://www.ncbi.nlm.nih.gov/pubmed/25990737
http://dx.doi.org/10.1093/nar/gkv521

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4499144/
https://www.ncbi.nlm.nih.gov/pubmed/25990737
http://dx.doi.org/10.1093/nar/gkv521

Ejemplares similares