Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris

BACKGROUND: Tagging proteins is a standard method facilitating protein detection, purification or targeting. When tagging a certain protein of interest, it is challenging to predict which tag will give optimal results and will not interfere with protein folding, activity or yields. Ideally, multiple...

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Detalles Bibliográficos
Autores principales: Vogl, Thomas, Ahmad, Mudassar, Krainer, Florian W, Schwab, Helmut, Glieder, Anton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501187/
https://www.ncbi.nlm.nih.gov/pubmed/26169367
http://dx.doi.org/10.1186/s12934-015-0293-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501187/
https://www.ncbi.nlm.nih.gov/pubmed/26169367
http://dx.doi.org/10.1186/s12934-015-0293-6

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