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Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris
BACKGROUND: Tagging proteins is a standard method facilitating protein detection, purification or targeting. When tagging a certain protein of interest, it is challenging to predict which tag will give optimal results and will not interfere with protein folding, activity or yields. Ideally, multiple...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501187/ https://www.ncbi.nlm.nih.gov/pubmed/26169367 http://dx.doi.org/10.1186/s12934-015-0293-6 |