Removal of Contaminant DNA by Combined UV-EMA Treatment Allows Low Copy Number Detection of Clinically Relevant Bacteria Using Pan-Bacterial Real-Time PCR

BACKGROUND: More than two decades after its discovery, contaminant microbial DNA in PCR reagents continues to impact the sensitivity and integrity of broad-range PCR diagnostic techniques. This is particularly relevant to their use in the setting of human sepsis, where a successful diagnostic on blo...

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Detalles Bibliográficos
Autores principales: Humphrey, Bruce, McLeod, Neil, Turner, Carrie, Sutton, J. Mark, Dark, Paul M., Warhurst, Geoffrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501569/
https://www.ncbi.nlm.nih.gov/pubmed/26172943
http://dx.doi.org/10.1371/journal.pone.0132954

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501569/
https://www.ncbi.nlm.nih.gov/pubmed/26172943
http://dx.doi.org/10.1371/journal.pone.0132954

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