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Sirtuin Inhibition Adversely Affects Porcine Oocyte Meiosis
Sirtuins have been implicated in diverse biological processes, including oxidative stress, energy metabolism, cell migration, and aging. Here, we employed Sirtuin inhibitors, nicotinamide (NAM) and Sirtinol, to investigate their effects on porcine oocyte maturation respectively. The rate of polar bo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503529/ https://www.ncbi.nlm.nih.gov/pubmed/26176547 http://dx.doi.org/10.1371/journal.pone.0132941 |
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author | Zhang, Liang Ma, Rujun Hu, Jin Ding, Xiaolin Xu, Yinxue |
author_facet | Zhang, Liang Ma, Rujun Hu, Jin Ding, Xiaolin Xu, Yinxue |
author_sort | Zhang, Liang |
collection | PubMed |
description | Sirtuins have been implicated in diverse biological processes, including oxidative stress, energy metabolism, cell migration, and aging. Here, we employed Sirtuin inhibitors, nicotinamide (NAM) and Sirtinol, to investigate their effects on porcine oocyte maturation respectively. The rate of polar body extrusion in porcine oocytes decreased after treatment with NAM and Sirtinol, accompanied with the failure of cumulus cell expansion. We further found that NAM and Sirtinol significantly disrupted oocyte polarity, and inhibited the formation of actin cap and cortical granule-free domain (CGFD). Moreover, the abnormal spindles and misaligned chromosomes were readily detected during porcine oocyte maturation after treatment with NAM and Sirtinol. Together, these results suggest that Sirtuins are involved in cortical polarity and spindle organization in porcine oocytes. |
format | Online Article Text |
id | pubmed-4503529 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45035292015-07-17 Sirtuin Inhibition Adversely Affects Porcine Oocyte Meiosis Zhang, Liang Ma, Rujun Hu, Jin Ding, Xiaolin Xu, Yinxue PLoS One Research Article Sirtuins have been implicated in diverse biological processes, including oxidative stress, energy metabolism, cell migration, and aging. Here, we employed Sirtuin inhibitors, nicotinamide (NAM) and Sirtinol, to investigate their effects on porcine oocyte maturation respectively. The rate of polar body extrusion in porcine oocytes decreased after treatment with NAM and Sirtinol, accompanied with the failure of cumulus cell expansion. We further found that NAM and Sirtinol significantly disrupted oocyte polarity, and inhibited the formation of actin cap and cortical granule-free domain (CGFD). Moreover, the abnormal spindles and misaligned chromosomes were readily detected during porcine oocyte maturation after treatment with NAM and Sirtinol. Together, these results suggest that Sirtuins are involved in cortical polarity and spindle organization in porcine oocytes. Public Library of Science 2015-07-15 /pmc/articles/PMC4503529/ /pubmed/26176547 http://dx.doi.org/10.1371/journal.pone.0132941 Text en © 2015 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zhang, Liang Ma, Rujun Hu, Jin Ding, Xiaolin Xu, Yinxue Sirtuin Inhibition Adversely Affects Porcine Oocyte Meiosis |
title | Sirtuin Inhibition Adversely Affects Porcine Oocyte Meiosis |
title_full | Sirtuin Inhibition Adversely Affects Porcine Oocyte Meiosis |
title_fullStr | Sirtuin Inhibition Adversely Affects Porcine Oocyte Meiosis |
title_full_unstemmed | Sirtuin Inhibition Adversely Affects Porcine Oocyte Meiosis |
title_short | Sirtuin Inhibition Adversely Affects Porcine Oocyte Meiosis |
title_sort | sirtuin inhibition adversely affects porcine oocyte meiosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503529/ https://www.ncbi.nlm.nih.gov/pubmed/26176547 http://dx.doi.org/10.1371/journal.pone.0132941 |
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