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A flexible and economical barcoding approach for highly multiplexed amplicon sequencing of diverse target genes

High throughput sequencing of phylogenetic and functional gene amplicons provides tremendous insight into the structure and functional potential of complex microbial communities. Here, we introduce a highly adaptable and economical PCR approach to barcoding and pooling libraries of numerous target g...

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Detalles Bibliográficos
Autores principales: Herbold, Craig W., Pelikan, Claus, Kuzyk, Orest, Hausmann, Bela, Angel, Roey, Berry, David, Loy, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503924/
https://www.ncbi.nlm.nih.gov/pubmed/26236305
http://dx.doi.org/10.3389/fmicb.2015.00731