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Bivalent separation into univalents precedes age-related meiosis I errors in oocytes

The frequency of chromosome segregation errors during meiosis I (MI) in oocytes increases with age. The two-hit model suggests that errors are caused by the combination of a first hit that creates susceptible crossover configurations and a second hit comprising an age-related reduction in chromosome...

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Autores principales: Sakakibara, Yogo, Hashimoto, Shu, Nakaoka, Yoshiharu, Kouznetsova, Anna, Höög, Christer, Kitajima, Tomoya S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507009/
https://www.ncbi.nlm.nih.gov/pubmed/26130582
http://dx.doi.org/10.1038/ncomms8550
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author Sakakibara, Yogo
Hashimoto, Shu
Nakaoka, Yoshiharu
Kouznetsova, Anna
Höög, Christer
Kitajima, Tomoya S.
author_facet Sakakibara, Yogo
Hashimoto, Shu
Nakaoka, Yoshiharu
Kouznetsova, Anna
Höög, Christer
Kitajima, Tomoya S.
author_sort Sakakibara, Yogo
collection PubMed
description The frequency of chromosome segregation errors during meiosis I (MI) in oocytes increases with age. The two-hit model suggests that errors are caused by the combination of a first hit that creates susceptible crossover configurations and a second hit comprising an age-related reduction in chromosome cohesion. This model predicts an age-related increase in univalents, but direct evidence of this phenomenon as a major cause of segregation errors has been lacking. Here, we provide the first live analysis of single chromosomes undergoing segregation errors during MI in the oocytes of naturally aged mice. Chromosome tracking reveals that 80% of the errors are preceded by bivalent separation into univalents. The set of the univalents is biased towards balanced and unbalanced predivision of sister chromatids during MI. Moreover, we find univalents predisposed to predivision in human oocytes. This study defines premature bivalent separation into univalents as the primary defect responsible for age-related aneuploidy.
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spelling pubmed-45070092015-07-21 Bivalent separation into univalents precedes age-related meiosis I errors in oocytes Sakakibara, Yogo Hashimoto, Shu Nakaoka, Yoshiharu Kouznetsova, Anna Höög, Christer Kitajima, Tomoya S. Nat Commun Article The frequency of chromosome segregation errors during meiosis I (MI) in oocytes increases with age. The two-hit model suggests that errors are caused by the combination of a first hit that creates susceptible crossover configurations and a second hit comprising an age-related reduction in chromosome cohesion. This model predicts an age-related increase in univalents, but direct evidence of this phenomenon as a major cause of segregation errors has been lacking. Here, we provide the first live analysis of single chromosomes undergoing segregation errors during MI in the oocytes of naturally aged mice. Chromosome tracking reveals that 80% of the errors are preceded by bivalent separation into univalents. The set of the univalents is biased towards balanced and unbalanced predivision of sister chromatids during MI. Moreover, we find univalents predisposed to predivision in human oocytes. This study defines premature bivalent separation into univalents as the primary defect responsible for age-related aneuploidy. Nature Pub. Group 2015-07-01 /pmc/articles/PMC4507009/ /pubmed/26130582 http://dx.doi.org/10.1038/ncomms8550 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Sakakibara, Yogo
Hashimoto, Shu
Nakaoka, Yoshiharu
Kouznetsova, Anna
Höög, Christer
Kitajima, Tomoya S.
Bivalent separation into univalents precedes age-related meiosis I errors in oocytes
title Bivalent separation into univalents precedes age-related meiosis I errors in oocytes
title_full Bivalent separation into univalents precedes age-related meiosis I errors in oocytes
title_fullStr Bivalent separation into univalents precedes age-related meiosis I errors in oocytes
title_full_unstemmed Bivalent separation into univalents precedes age-related meiosis I errors in oocytes
title_short Bivalent separation into univalents precedes age-related meiosis I errors in oocytes
title_sort bivalent separation into univalents precedes age-related meiosis i errors in oocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507009/
https://www.ncbi.nlm.nih.gov/pubmed/26130582
http://dx.doi.org/10.1038/ncomms8550
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