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QD-Based FRET Probes at a Glance
The unique optoelectronic properties of quantum dots (QDs) give them significant advantages over traditional organic dyes, not only as fluorescent labels for bioimaging, but also as emissive sensing probes. QD sensors that function via manipulation of fluorescent resonance energy transfer (FRET) are...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507597/ https://www.ncbi.nlm.nih.gov/pubmed/26053750 http://dx.doi.org/10.3390/s150613028 |
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author | Shamirian, Armen Ghai, Aashima Snee, Preston T. |
author_facet | Shamirian, Armen Ghai, Aashima Snee, Preston T. |
author_sort | Shamirian, Armen |
collection | PubMed |
description | The unique optoelectronic properties of quantum dots (QDs) give them significant advantages over traditional organic dyes, not only as fluorescent labels for bioimaging, but also as emissive sensing probes. QD sensors that function via manipulation of fluorescent resonance energy transfer (FRET) are of special interest due to the multiple response mechanisms that may be utilized, which in turn imparts enhanced flexibility in their design. They may also function as ratiometric, or “color-changing” probes. In this review, we describe the fundamentals of FRET and provide examples of QD-FRET sensors as grouped by their response mechanisms such as link cleavage and structural rearrangement. An overview of early works, recent advances, and various models of QD-FRET sensors for the measurement of pH and oxygen, as well as the presence of metal ions and proteins such as enzymes, are also provided. |
format | Online Article Text |
id | pubmed-4507597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-45075972015-07-22 QD-Based FRET Probes at a Glance Shamirian, Armen Ghai, Aashima Snee, Preston T. Sensors (Basel) Review The unique optoelectronic properties of quantum dots (QDs) give them significant advantages over traditional organic dyes, not only as fluorescent labels for bioimaging, but also as emissive sensing probes. QD sensors that function via manipulation of fluorescent resonance energy transfer (FRET) are of special interest due to the multiple response mechanisms that may be utilized, which in turn imparts enhanced flexibility in their design. They may also function as ratiometric, or “color-changing” probes. In this review, we describe the fundamentals of FRET and provide examples of QD-FRET sensors as grouped by their response mechanisms such as link cleavage and structural rearrangement. An overview of early works, recent advances, and various models of QD-FRET sensors for the measurement of pH and oxygen, as well as the presence of metal ions and proteins such as enzymes, are also provided. MDPI 2015-06-04 /pmc/articles/PMC4507597/ /pubmed/26053750 http://dx.doi.org/10.3390/s150613028 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Shamirian, Armen Ghai, Aashima Snee, Preston T. QD-Based FRET Probes at a Glance |
title | QD-Based FRET Probes at a Glance |
title_full | QD-Based FRET Probes at a Glance |
title_fullStr | QD-Based FRET Probes at a Glance |
title_full_unstemmed | QD-Based FRET Probes at a Glance |
title_short | QD-Based FRET Probes at a Glance |
title_sort | qd-based fret probes at a glance |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507597/ https://www.ncbi.nlm.nih.gov/pubmed/26053750 http://dx.doi.org/10.3390/s150613028 |
work_keys_str_mv | AT shamirianarmen qdbasedfretprobesataglance AT ghaiaashima qdbasedfretprobesataglance AT sneeprestont qdbasedfretprobesataglance |