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QD-Based FRET Probes at a Glance

The unique optoelectronic properties of quantum dots (QDs) give them significant advantages over traditional organic dyes, not only as fluorescent labels for bioimaging, but also as emissive sensing probes. QD sensors that function via manipulation of fluorescent resonance energy transfer (FRET) are...

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Detalles Bibliográficos
Autores principales: Shamirian, Armen, Ghai, Aashima, Snee, Preston T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507597/
https://www.ncbi.nlm.nih.gov/pubmed/26053750
http://dx.doi.org/10.3390/s150613028
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author Shamirian, Armen
Ghai, Aashima
Snee, Preston T.
author_facet Shamirian, Armen
Ghai, Aashima
Snee, Preston T.
author_sort Shamirian, Armen
collection PubMed
description The unique optoelectronic properties of quantum dots (QDs) give them significant advantages over traditional organic dyes, not only as fluorescent labels for bioimaging, but also as emissive sensing probes. QD sensors that function via manipulation of fluorescent resonance energy transfer (FRET) are of special interest due to the multiple response mechanisms that may be utilized, which in turn imparts enhanced flexibility in their design. They may also function as ratiometric, or “color-changing” probes. In this review, we describe the fundamentals of FRET and provide examples of QD-FRET sensors as grouped by their response mechanisms such as link cleavage and structural rearrangement. An overview of early works, recent advances, and various models of QD-FRET sensors for the measurement of pH and oxygen, as well as the presence of metal ions and proteins such as enzymes, are also provided.
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spelling pubmed-45075972015-07-22 QD-Based FRET Probes at a Glance Shamirian, Armen Ghai, Aashima Snee, Preston T. Sensors (Basel) Review The unique optoelectronic properties of quantum dots (QDs) give them significant advantages over traditional organic dyes, not only as fluorescent labels for bioimaging, but also as emissive sensing probes. QD sensors that function via manipulation of fluorescent resonance energy transfer (FRET) are of special interest due to the multiple response mechanisms that may be utilized, which in turn imparts enhanced flexibility in their design. They may also function as ratiometric, or “color-changing” probes. In this review, we describe the fundamentals of FRET and provide examples of QD-FRET sensors as grouped by their response mechanisms such as link cleavage and structural rearrangement. An overview of early works, recent advances, and various models of QD-FRET sensors for the measurement of pH and oxygen, as well as the presence of metal ions and proteins such as enzymes, are also provided. MDPI 2015-06-04 /pmc/articles/PMC4507597/ /pubmed/26053750 http://dx.doi.org/10.3390/s150613028 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Shamirian, Armen
Ghai, Aashima
Snee, Preston T.
QD-Based FRET Probes at a Glance
title QD-Based FRET Probes at a Glance
title_full QD-Based FRET Probes at a Glance
title_fullStr QD-Based FRET Probes at a Glance
title_full_unstemmed QD-Based FRET Probes at a Glance
title_short QD-Based FRET Probes at a Glance
title_sort qd-based fret probes at a glance
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507597/
https://www.ncbi.nlm.nih.gov/pubmed/26053750
http://dx.doi.org/10.3390/s150613028
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