Nested PCR Biases in Interpreting Microbial Community Structure in 16S rRNA Gene Sequence Datasets

BACKGROUND: Sequencing of the PCR-amplified 16S rRNA gene has become a common approach to microbial community investigations in the fields of human health and environmental sciences. This approach, however, is difficult when the amount of DNA is too low to be amplified by standard PCR. Nested PCR ca...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Guoqin, Fadrosh, Doug, Goedert, James J., Ravel, Jacques, Goldstein, Alisa M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4509648/
https://www.ncbi.nlm.nih.gov/pubmed/26196512
http://dx.doi.org/10.1371/journal.pone.0132253

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4509648/
https://www.ncbi.nlm.nih.gov/pubmed/26196512
http://dx.doi.org/10.1371/journal.pone.0132253

Ejemplares similares