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Nested PCR Biases in Interpreting Microbial Community Structure in 16S rRNA Gene Sequence Datasets
BACKGROUND: Sequencing of the PCR-amplified 16S rRNA gene has become a common approach to microbial community investigations in the fields of human health and environmental sciences. This approach, however, is difficult when the amount of DNA is too low to be amplified by standard PCR. Nested PCR ca...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4509648/ https://www.ncbi.nlm.nih.gov/pubmed/26196512 http://dx.doi.org/10.1371/journal.pone.0132253 |