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Eicosapentaenoic and docosahexaenoic acid-enriched high fat diet delays the development of fatty liver in mice

BACKGROUND: Low hepatic content of n-3 PUFA has been associated with NAFLD in humans. Whether this is associated with reduced dietary intake or increased turnover of these FA is not clear. We have here investigated the effects of dietary fat quality on hepatic lipid storage and transcriptomics over...

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Autores principales: Soni, Nikul K, Nookaew, Intawat, Sandberg, Ann-Sofie, Gabrielsson, Britt G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4509768/
https://www.ncbi.nlm.nih.gov/pubmed/26193881
http://dx.doi.org/10.1186/s12944-015-0072-8
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author Soni, Nikul K
Nookaew, Intawat
Sandberg, Ann-Sofie
Gabrielsson, Britt G
author_facet Soni, Nikul K
Nookaew, Intawat
Sandberg, Ann-Sofie
Gabrielsson, Britt G
author_sort Soni, Nikul K
collection PubMed
description BACKGROUND: Low hepatic content of n-3 PUFA has been associated with NAFLD in humans. Whether this is associated with reduced dietary intake or increased turnover of these FA is not clear. We have here investigated the effects of dietary fat quality on hepatic lipid storage and transcriptomics over time. AIM: To investigate the effects of quality of fat in a high fat diet (HFD) over time on hepatic lipid storage and liver transcriptomics. METHODS AND RESULTS: Male C57BL/6J mice were fed control, HFD-eicosapentaenoic acid (EPA)/ docosahexaenoic acid (DHA) or HFD-corn oil diet for 8 or 12 weeks. Body weight, body composition, plasma and hepatic triglyceride contents were measured. Hepatic transcriptomes were analysed by microarray followed by gene-set enrichment analyses. At 8 weeks, the HFD-corn oil mice had higher body weight and adipose depot mass than the HFD-EPA/DHA but there were no differences at 12 weeks. Hepatic triglyceride content was lower in HFD-EPA/DHA fed compared with the HFD-corn oil fed mice at both time-points. Enrichment analyses of the hepatic transcriptomes showed that lipid/fatty acid biosynthesis; transport and homeostasis were lower in the HFD-EPA/DHA fed compared with the HFD-corn oil fed mice. Genes encoding proteins associated to cytoplasmic lipid droplets were expressed at higher levels in livers from the HFD-corn oil compared to HFD-EPA/DHA mice. CONCLUSIONS: Dietary EPA and DHA counteracted development of HFD-induced fatty liver. The liver transcriptome data implicate that the quality of dietary fat could modulate Ppar-related gene expression that in turn affects hepatic lipid storage and maintenance of metabolic health. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-015-0072-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-45097682015-07-23 Eicosapentaenoic and docosahexaenoic acid-enriched high fat diet delays the development of fatty liver in mice Soni, Nikul K Nookaew, Intawat Sandberg, Ann-Sofie Gabrielsson, Britt G Lipids Health Dis Research BACKGROUND: Low hepatic content of n-3 PUFA has been associated with NAFLD in humans. Whether this is associated with reduced dietary intake or increased turnover of these FA is not clear. We have here investigated the effects of dietary fat quality on hepatic lipid storage and transcriptomics over time. AIM: To investigate the effects of quality of fat in a high fat diet (HFD) over time on hepatic lipid storage and liver transcriptomics. METHODS AND RESULTS: Male C57BL/6J mice were fed control, HFD-eicosapentaenoic acid (EPA)/ docosahexaenoic acid (DHA) or HFD-corn oil diet for 8 or 12 weeks. Body weight, body composition, plasma and hepatic triglyceride contents were measured. Hepatic transcriptomes were analysed by microarray followed by gene-set enrichment analyses. At 8 weeks, the HFD-corn oil mice had higher body weight and adipose depot mass than the HFD-EPA/DHA but there were no differences at 12 weeks. Hepatic triglyceride content was lower in HFD-EPA/DHA fed compared with the HFD-corn oil fed mice at both time-points. Enrichment analyses of the hepatic transcriptomes showed that lipid/fatty acid biosynthesis; transport and homeostasis were lower in the HFD-EPA/DHA fed compared with the HFD-corn oil fed mice. Genes encoding proteins associated to cytoplasmic lipid droplets were expressed at higher levels in livers from the HFD-corn oil compared to HFD-EPA/DHA mice. CONCLUSIONS: Dietary EPA and DHA counteracted development of HFD-induced fatty liver. The liver transcriptome data implicate that the quality of dietary fat could modulate Ppar-related gene expression that in turn affects hepatic lipid storage and maintenance of metabolic health. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-015-0072-8) contains supplementary material, which is available to authorized users. BioMed Central 2015-07-22 /pmc/articles/PMC4509768/ /pubmed/26193881 http://dx.doi.org/10.1186/s12944-015-0072-8 Text en © Soni et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Soni, Nikul K
Nookaew, Intawat
Sandberg, Ann-Sofie
Gabrielsson, Britt G
Eicosapentaenoic and docosahexaenoic acid-enriched high fat diet delays the development of fatty liver in mice
title Eicosapentaenoic and docosahexaenoic acid-enriched high fat diet delays the development of fatty liver in mice
title_full Eicosapentaenoic and docosahexaenoic acid-enriched high fat diet delays the development of fatty liver in mice
title_fullStr Eicosapentaenoic and docosahexaenoic acid-enriched high fat diet delays the development of fatty liver in mice
title_full_unstemmed Eicosapentaenoic and docosahexaenoic acid-enriched high fat diet delays the development of fatty liver in mice
title_short Eicosapentaenoic and docosahexaenoic acid-enriched high fat diet delays the development of fatty liver in mice
title_sort eicosapentaenoic and docosahexaenoic acid-enriched high fat diet delays the development of fatty liver in mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4509768/
https://www.ncbi.nlm.nih.gov/pubmed/26193881
http://dx.doi.org/10.1186/s12944-015-0072-8
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