Selective transcriptional regulation by Myc: Experimental design and computational analysis of high-throughput sequencing data

The gene expression programs regulated by the Myc transcription factor were evaluated by integrated genome-wide profiling of Myc binding sites, chromatin marks and RNA expression in several biological models. Our results indicate that Myc directly drives selective transcriptional regulation, which i...

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Detalles Bibliográficos
Autores principales: Pelizzola, Mattia, Morelli, Marco J., Sabò, Arianna, Kress, Theresia R., de Pretis, Stefano, Amati, Bruno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510069/
https://www.ncbi.nlm.nih.gov/pubmed/26217715
http://dx.doi.org/10.1016/j.dib.2015.02.003
Descripción
Sumario:The gene expression programs regulated by the Myc transcription factor were evaluated by integrated genome-wide profiling of Myc binding sites, chromatin marks and RNA expression in several biological models. Our results indicate that Myc directly drives selective transcriptional regulation, which in certain physiological conditions may indirectly lead to RNA amplification. Here, we illustrate in detail the experimental design concerning the high-throughput sequencing data associated with our study (Sabò et al., Nature. (2014) 511:488–492) and the R scripts used for their computational analysis.

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