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Data supporting the design and evaluation of a universal primer pair for pseudogene-free amplification of HPRT1 in real-time PCR

Hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) is a common housekeeping gene for sample normalization in the quantitative reverse transcriptase polymerase chain (qRT-PCR). However, co-amplification of HPRT1 pseudogenes may affect accurate results obtained in qRT-PCR. We designed a primer p...

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Detalles Bibliográficos
Autores principales: Valadan, Reza, Hedayatizadeh-Omran, Akbar, Alhosseini-Abyazani, Mahdyieh Naghavi, Amjadi, Omolbanin, Rafiei, Alireza, Tehrani, Mohsen, Alizadeh-Navaei, Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510554/
https://www.ncbi.nlm.nih.gov/pubmed/26217821
http://dx.doi.org/10.1016/j.dib.2015.06.009