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Myristoylation profiling in human cells and zebrafish

Human cells (HEK 293, HeLa, MCF-7) and zebrafish embryos were metabolically tagged with an alkynyl myristic acid probe, lysed with an SDS buffer and tagged proteomes ligated to multifunctional capture reagents via copper-catalyzed alkyne azide cycloaddition (CuAAC). This allowed for affinity enrichm...

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Detalles Bibliográficos
Autores principales: Broncel, Malgorzata, Serwa, Remigiusz A, Ciepla, Paulina, Krause, Eberhard, Dallman, Margaret J., Magee, Anthony I., Tate, Edward W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510570/
https://www.ncbi.nlm.nih.gov/pubmed/26217820
http://dx.doi.org/10.1016/j.dib.2015.06.010