CHO-S Antibody Titers >1 Gram/Liter Using Flow Electroporation-Mediated Transient Gene Expression followed by Rapid Migration to High-Yield Stable Cell Lines

In recent years, researchers have turned to transient gene expression (TGE) as an alternative to CHO stable cell line generation for early-stage antibody development. Despite advances in transfection methods and culture optimization, the majority of CHO-based TGE systems produce insufficient antibod...

Descripción completa

Detalles Bibliográficos
Autores principales: Steger, Krista, Brady, James, Wang, Weili, Duskin, Meg, Donato, Karen, Peshwa, Madhusudan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2015
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512034/
https://www.ncbi.nlm.nih.gov/pubmed/25520372
http://dx.doi.org/10.1177/1087057114563494

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512034/
https://www.ncbi.nlm.nih.gov/pubmed/25520372
http://dx.doi.org/10.1177/1087057114563494

Ejemplares similares