A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases

A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.

Detalles Bibliográficos
Autores principales: Angelini, Leticia Mara Lima, da Silva, Amanda Ribeiro Martins, Rocco, Lucas de Freitas Coli, Milagre, Cintia Duarte de Freitas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2015
Materias:
Environmental Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512080/
https://www.ncbi.nlm.nih.gov/pubmed/26221095
http://dx.doi.org/10.1590/S1517-838246120130851

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512080/
https://www.ncbi.nlm.nih.gov/pubmed/26221095
http://dx.doi.org/10.1590/S1517-838246120130851

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