A pre-screening FISH-based method to detect CRISPR/Cas9 off-targets in mouse embryonic stem cells

The clustered regularly interspaced short palindromic repeat (CRISPR)/associated 9 (Cas9) technology has been recently added to the tools allowing efficient and easy DNA targeting, representing a very promising approach to gene engineering. Using the CRISPR/Cas9 system we have driven the integration...

Descripción completa

Detalles Bibliográficos
Autores principales: Paulis, Marianna, Castelli, Alessandra, Lizier, Michela, Susani, Lucia, Lucchini, Franco, Villa, Anna, Vezzoni, Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513284/
https://www.ncbi.nlm.nih.gov/pubmed/26204993
http://dx.doi.org/10.1038/srep12327

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513284/
https://www.ncbi.nlm.nih.gov/pubmed/26204993
http://dx.doi.org/10.1038/srep12327

Ejemplares similares