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A pre-screening FISH-based method to detect CRISPR/Cas9 off-targets in mouse embryonic stem cells

The clustered regularly interspaced short palindromic repeat (CRISPR)/associated 9 (Cas9) technology has been recently added to the tools allowing efficient and easy DNA targeting, representing a very promising approach to gene engineering. Using the CRISPR/Cas9 system we have driven the integration...

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Detalles Bibliográficos
Autores principales:	Paulis, Marianna, Castelli, Alessandra, Lizier, Michela, Susani, Lucia, Lucchini, Franco, Villa, Anna, Vezzoni, Paolo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2015
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513284/ https://www.ncbi.nlm.nih.gov/pubmed/26204993 http://dx.doi.org/10.1038/srep12327

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