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A set of powerful negative selection systems for unmodified Enterobacteriaceae

Creation of defined genetic mutations is a powerful method for dissecting mechanisms of bacterial disease; however, many genetic tools are only developed for laboratory strains. We have designed a modular and general negative selection strategy based on inducible toxins that provides high selection...

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Detalles Bibliográficos
Autores principales: Khetrapal, Varnica, Mehershahi, Kurosh, Rafee, Shazmina, Chen, Siyi, Lim, Chiew Ling, Chen, Swaine L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513841/
https://www.ncbi.nlm.nih.gov/pubmed/25800749
http://dx.doi.org/10.1093/nar/gkv248